期刊论文详细信息
Journal of Neuroinflammation | |
Neural precursor cell delivery induces acute post-ischemic cerebroprotection, but fails to promote long-term stroke recovery in hyperlipidemic mice due to mechanisms that include pro-inflammatory responses associated with brain hemorrhages | |
Research | |
Dirk M. Hermann1  Dongpei Yin1  Yachao Qi1  Chen Wang1  Nina Hagemann1  Ayan Mohamud Yusuf1  Egor Dzyubenko1  Britta Kaltwasser1  Aurel Popa-Wagner2  Thorsten R. Doeppner3  Ya-Chao Wang4  Matthias Gunzer5  Bernd Giebel6  Tobias Tertel6  | |
[1]Department of Neurology, University Hospital Essen, University of Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Germany | |
[2]Department of Neurology, University Hospital Essen, University of Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Germany | |
[3]Center of Experimental and Clinical Medicine, University of Medicine and Pharmacy, Craiova, Romania | |
[4]Department of Neurology, University Hospital Essen, University of Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Germany | |
[5]Department of Neurology, Justus-Liebig University Gießen, Giessen, Germany | |
[6]Department of Neurology, University Hospital Essen, University of Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Germany | |
[7]Institute of Translational Medicine, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, China | |
[8]Institute for Experimental Immunology and Imaging and Imaging Center Essen (IMCES), University Hospital Essen, University of Duisburg-Essen, Essen, Germany | |
[9]Leibniz-Institut für Analytische Wissenschaften –ISAS– e.V., Dortmund, Germany | |
[10]Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, Germany | |
关键词: Anti-inflammation; Cholesterol-rich diet; Focal cerebral ischemia; Hypercholesterolemia; Light sheet microscopy; Microglia/macrophage morphology; Middle cerebral artery occlusion; Neuroblast; Neural stem cell; Western diet; | |
DOI : 10.1186/s12974-023-02894-8 | |
received in 2023-08-02, accepted in 2023-09-07, 发布年份 2023 | |
来源: Springer | |
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【 摘 要 】
BackgroundThe intravenous delivery of adult neural precursor cells (NPC) has shown promising results in enabling cerebroprotection, brain tissue remodeling, and neurological recovery in young, healthy stroke mice. However, the translation of cell-based therapies to clinical settings has encountered challenges. It remained unclear if adult NPCs could induce brain tissue remodeling and recovery in mice with hyperlipidemia, a prevalent vascular risk factor in stroke patients.MethodsMale mice on a normal (regular) diet or on cholesterol-rich Western diet were exposed to 30 min intraluminal middle cerebral artery occlusion (MCAO). Vehicle or 106 NPCs were intravenously administered immediately after reperfusion, at 3 day and 7 day post-MCAO. Neurological recovery was evaluated using the Clark score, Rotarod and tight rope tests over up to 56 days. Histochemistry and light sheet microscopy were used to examine ischemic injury and brain tissue remodeling. Immunological responses in peripheral blood and brain were analyzed through flow cytometry.ResultsNPC administration reduced infarct volume, blood–brain barrier permeability and the brain infiltration of neutrophils, monocytes, T cells and NK cells in the acute stroke phase in both normolipidemic and hyperlipidemic mice, but increased brain hemorrhage formation and neutrophil, monocyte and CD4+ and CD8+ T cell counts and activation in the blood of hyperlipidemic mice. While neurological deficits in hyperlipidemic mice were reduced by NPCs at 3 day post-MCAO, NPCs did not improve neurological deficits at later timepoints. Besides, NPCs did not influence microglia/macrophage abundance and activation (assessed by morphology analysis), astroglial scar formation, microvascular length or branching point density (evaluated using light sheet microscopy), long-term neuronal survival or brain atrophy in hyperlipidemic mice.ConclusionsIntravenously administered NPCs did not have persistent effects on post-ischemic neurological recovery and brain remodeling in hyperlipidemic mice. These findings highlight the necessity of rigorous investigations in vascular risk factor models to fully assess the long-term restorative effects of cell-based therapies. Without comprehensive studies in such models, the clinical potential of cell-based therapies cannot be definitely determined.【 授权许可】
CC BY
© BioMed Central Ltd., part of Springer Nature 2023
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【 参考文献 】
- [1]
- [2]
- [3]
- [4]
- [5]
- [6]
- [7]
- [8]
- [9]
- [10]
- [11]
- [12]
- [13]
- [14]
- [15]
- [16]
- [17]
- [18]
- [19]
- [20]
- [21]
- [22]
- [23]
- [24]
- [25]
- [26]
- [27]
- [28]
- [29]
- [30]
- [31]
- [32]
- [33]
- [34]
- [35]
- [36]
- [37]
- [38]
- [39]
- [40]
- [41]