Synthesis of isobutanol using acetate as sole carbon source in Escherichia coli

Microbial Cell Factories

Research

Shuixing Jiang¹  Chengwei Li¹  Hao Zhou¹  Hao Niu²  Qiang Li²  Shuo Zhao²  Pengfei Gu² 

[1] RZBC GROUP CO., LTD, 276800, Rizhao, Shandong, China;School of Biological Science and Technology, University of Jinan, 250022, Jinan, People’s Republic of China;

关键词: Isobutanol;    Escherichia coli;    Acetate;    Metabolic engineering;    Pyruvate;   

DOI  :  10.1186/s12934-023-02197-w

 received in 2023-03-26, accepted in 2023-09-06,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundWith concerns about depletion of fossil fuel and environmental pollution, synthesis of biofuels such as isobutanol from low-cost substrate by microbial cell factories has attracted more and more attention. As one of the most promising carbon sources instead of food resources, acetate can be utilized by versatile microbes and converted into numerous valuable chemicals.ResultsAn isobutanol synthetic pathway using acetate as sole carbon source was constructed in E. coli. Pyruvate was designed to be generated via acetyl-CoA by pyruvate-ferredoxin oxidoreductase YdbK or anaplerotic pathway. Overexpression of transhydrogenase and NAD kinase increased the isobutanol titer of recombinant E. coli from 121.21 mg/L to 131.5 mg/L under batch cultivation. Further optimization of acetate supplement concentration achieved 157.05 mg/L isobutanol accumulation in WY002, representing the highest isobutanol titer by using acetate as sole carbon source.ConclusionsThe utilization of acetate as carbon source for microbial production of valuable chemicals such as isobutanol could reduce the consumption of food-based substrates and save production cost. Engineering strategies applied in this study will provide a useful reference for microbial production of pyruvate derived chemical compounds from acetate.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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