New mechanisms and biomarkers of lymph node metastasis in cervical cancer: reflections from plasma proteomics

Clinical Proteomics

Research

Jingjing Zhang¹  Shuang Ju¹  Gulijinaiti Abulikemu¹  Yi Li¹  Baoxia Cui¹  Hongli Liu¹  Qian Chen¹  Xiaoli Liu¹  Wendi Mu¹  Youzhong Zhang¹  Jiaqi Yang¹  Qianwei Zhen¹  Sai Han¹  Shuxia Wu² 

[1] Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, 107 Wenhua Xi Road, 250012, Jinan, Shandong, People’s Republic of China;Department of Obstetrics and Gynecology, the Fifth People’s Hospital of Jinan, 250012, Jinan, Shandong, People’s Republic of China;

关键词: DIA;    Cervical cancer;    LVSI;    LNM;    Biomarker;   

DOI  :  10.1186/s12014-023-09427-8

 received in 2022-10-26, accepted in 2023-08-21,  发布年份 2023

来源: Springer

【 摘 要 】

ObjectiveLymph node metastasis (LNM) and lymphatic vasculature space infiltration (LVSI) in cervical cancer patients indicate a poor prognosis, but satisfactory methods for diagnosing these phenotypes are lacking. This study aimed to find new effective plasma biomarkers of LNM and LVSI as well as possible mechanisms underlying LNM and LVSI through data-independent acquisition (DIA) proteome sequencing.MethodsA total of 20 cervical cancer plasma samples, including 7 LNM-/LVSI-(NC), 4 LNM-/LVSI + (LVSI) and 9 LNM + /LVSI + (LNM) samples from a cohort, were subjected to DIA to identify differentially expressed proteins (DEPs) for LVSI and LNM. Subsequently, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed for DEP functional annotation. Protein–protein interaction (PPI) and weighted gene coexpression network analysis (WGCNA) were used to detect new effective plasma biomarkers and possible mechanisms.ResultsA total of 79 DEPs were identified in the cohort. GO and KEGG analyses showed that DEPs were mainly enriched in the complement and coagulation pathway, lipid and atherosclerosis pathway, HIF-1 signal transduction pathway and phagosome and autophagy. WGCNA showed that the enrichment of the green module differed greatly between groups. Six interesting core DEPs (SPARC, HPX, VCAM1, TFRC, ERN1 and APMAP) were confirmed to be potential plasma diagnostic markers for LVSI and LNM in cervical cancer patients.ConclusionProteomic signatures developed in this study reflected the potential plasma diagnostic markers and new possible pathogenesis mechanisms in the LVSI and LNM of cervical cancer.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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