Microalgal upgrading of the fermentative biohydrogen produced from Bacillus coagulans via non-pretreated plant biomass

Microbial Cell Factories

Research

Aya H. A. Mahmoud¹  Maysa M. Ali¹  Ahmed A. Shoreit¹  Eman S. E. Aldaby¹  Asmaa M. M. Mawad²  Haitham M. El-Bery³ 

[1] Botany and Microbiology Department, Faculty of Science, Assiut University, 71515, Assiut, Egypt;Botany and Microbiology Department, Faculty of Science, Assiut University, 71515, Assiut, Egypt;Department of Biology, College of Science, Taibah University, 42317-8599, Madinah, Saudi Arabia;Green Hydrogen Production Laboratory, Chemistry Department, Faculty of Science, Assiut University, 71515, Assiut, Egypt;

关键词: Bacillus;    Biohydrogen;    CO sequestration;    Fermentation;    Microalgae;    Upgrading;   

DOI  :  10.1186/s12934-023-02193-0

 received in 2023-07-30, accepted in 2023-09-04,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundHydrogen is a promising source of alternative energy. Fermentative production is more feasible because of its high hydrogen generation rate, simple operating conditions, and utilization of various organic wastes as substrates. The most significant constraint for biohydrogen production is supplying it at a low cost with fewer impurities.ResultsLeaf biomass of Calotropis procera was used as a feedstock for a dark fermentative production of hydrogen by Bacillus coagulans AH1 (MN923076). The optimum operation conditions for biohydrogen production were 5.0% substrate concentrationand pH 9.0, at 35 °C. In which the biohydrogen yield was 3.231 mmol H2/g dry biomass without any pretreatments of the biomass. A freshwater microalga Oscillatroia sp was used for upgrading of the produced biohydrogen. It sequestrated 97 and 99% % of CO2 from the gas mixture when it was cultivated in BG11 and BG11-N media, respectively After upgrading process, the residual microalgal cells exhibited 0.21mg/mL of biomass yield,high content of chlorophyll-a (4.8 µg/mL) and carotenoid (11.1 µg/mL). In addition to Oscillatroia sp residual biomass showed a lipid yield (7.5–8.7%) on the tested media.ConclusionBacillus coagulans AH1 is a promising tool for biohydrogen production avoiding the drawbacks of biomass pretreatment. Oscillatroia sp is encouraged as a potent tool for upgrading and purification of biohydrogen. These findings led to the development of a multiproduct biorefinery with zero waste that is more economically sustainable.Graphical Abstract

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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