Silver nanoparticles–decorated extracellular matrix graft: fabrication and tendon reconstruction performance

Biomaterials Research

Research Article

Qi Dong¹  Wei Yuan²  Xiaogang Bao³  Bing Wu⁴  Gaoxiang Ma⁴  Chennan Xu⁴  Sunfang Chen⁵  Bin Fang⁵  Dan Cai⁶ 

[1] Department of Orthopedics, Honghui Hospital, Xi’an Jiao Tong University, 710054, Xi’an City, China;Department of Orthopedics, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, 200071, Shanghai, China;Department of Orthopedics, The Spine Surgical Center, Second Affiliated Hospital of Naval Medical University, 200003, Shanghai, China;Department of Orthopedics, the Central Hospital Affiliated to Shaoxing University, 312030, Shaoxing, China;Department of Orthopedics, the First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), 310000, Hangzhou, China;Department of Orthopedics, the Central Hospital Affiliated to Shaoxing University, 312030, Shaoxing, China;Department of Orthopedics, the First People’s Hospital of Huzhou, First Affiliated Hospital of Huzhou University, 313000, Huzhou, China;

关键词: Tendon graft;    Extracellular matrix;    Silver nanoparticles;    Infection;    Inflammation;   

DOI  :  10.1186/s40824-023-00428-0

 received in 2023-06-07, accepted in 2023-09-03,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundThe reconstruction of tendons with large defects requires grafts with high mechanical strength and is often hindered by complications such as infection and adhesion. Hence, grafts combining the advantages of mechanical resilience and antibacterial/antiadhesion activity are highly sought after.MethodsThe silver nanoparticles (GA-Ag NPs) synthesized from gallic acid and silver nitrate were attached to a decellularized extracellular matrix (Decellularized Tendon crosslinking GA-AgNPs, DT-Ag). We examined the histological structure, mechanical property, morphology, Zeta potential, cytotoxicity, antibacterial properties, antioxidant and anti-inflammatory properties, and ability of the DT-Ag to treat tendon defects in animals.ResultsApproximately 108.57 ± 0.94 μg GA-Ag NPs loaded per 50 mg DT, the cross-linked part of GA-Ag NPs was 65.47 ± 0.57%, which provided DT-Ag with long-lasting antibacterial activity. Meanwhile, GA endowed DT-Ag with good antioxidant and anti-inflammatory activities. Additionally, The DT-Ag facilitated M2 macrophage polarization, and suppressed fibrin deposition by hindering fibroblast adhesion. Mormore, the main advantages of DT-Ag, namely its long-lasting antibacterial activity (tested using Escherichia coli and Staphylococcus aureus as models) and the ability to prevent tissue adhesion were confirmed in vivo.ConclusionThe fabricated multifunctional tendon graft was highly hydrophilic, biocompatible, and mechanically resilient, and concluded to be well suited for dealing with the main complications of surgical tendon reconstruction and has bright application prospects.Graphical Abstract

【 授权许可】

CC BY   
© The Korean Society for Biomaterials 2023

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