Artificial switches induce the bespoke production of functional compounds in marine microalgae Chlorella by neutralizing CO2

Biotechnology for Biofuels and Bioproducts

Research

Kalyanee Paithoonrangsarid¹  Xinyu Cui²  Jiahua Gu²  Mingcan Wu²  Yi Xin²  Aoqi Wang²  Yuan Xiao²  Yandu Lu³ 

[1] Biochemical Engineering and Systems Biology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, King Mongkut’s University of Technology Thonburi, Bangkok, Thailand;Single-cell BioEngineering Group, State Key Laboratory of Marine Resource Utilization in South China Sea, School of Marine Biology and Fisheries, Hainan University, 570228, Haikou, China;Single-cell BioEngineering Group, State Key Laboratory of Marine Resource Utilization in South China Sea, School of Marine Biology and Fisheries, Hainan University, 570228, Haikou, China;Hainan Provincial Key Laboratory of Tropical Hydrobiotechnology, Hainan University, Haikou, China;Haikou Technology Innovation Center for Research and Utilization of Algal Bioresources, Hainan University, Haikou, China;

关键词: Chlorella;    CO tolerance;    Microalgae;    Multiomics;    Mutant library;    Lipids;   

DOI  :  10.1186/s13068-023-02381-5

 received in 2023-05-19, accepted in 2023-08-11,  发布年份 2023

来源: Springer

【 摘 要 】

To improve the CO2 tolerance of a marine microalga Chlorella sp. of which the production capacity has been demonstrated industrially, a mutant library was created and a strain hct53 was screened. Compared to the parental strain, hct53 shows a high CO2 capture capacity, while starch biosynthesis is compromised, with increases in health beneficial metabolites and antioxidant capacity. Global gene expression and genome-wide mutation distribution revealed that transcript choreography was concomitant with more active CO2 sequestration, an increase in the lipid synthesis, and a decrease in the starch and protein synthesis. These results suggest that artificial trait improvement via mutagenesis, couple with multiomics analysis, helps discover genetic switches that induce the bespoke conversion of carbon flow from “redundant metabolites” to valuable ones for functional food.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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