Improved production of andrimid in Erwinia persicina BST187 strain by fermentation optimization

BMC Microbiology

Research

Lunqiang Zhao¹  Jianye Xia²  Yuyong Hou²  Tongling Ge²  Lei Zhao³  Tingfeng Cheng⁴ 

[1] Key Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, 300308, Tianjin, China;College of Biological Sciences, China Agricultural University, Beijing, China;Key Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, 300308, Tianjin, China;National Center of Technology Innovation for Synthetic Biology, Tianjin, China;Key Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, 300308, Tianjin, China;National Center of Technology Innovation for Synthetic Biology, Tianjin, China;College of Biological Sciences, China Agricultural University, Beijing, China;Key Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, 300308, Tianjin, China;University of Chinese Academy of Sciences, Beijing, China;

关键词: Andrimid;    Erwinia persicina;    Antibacterial activity;    Fermentation;    Design of experiment;    Response surface methodology;   

DOI  :  10.1186/s12866-023-02946-2

 received in 2023-04-19, accepted in 2023-07-14,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundAndrimid is reported to be a novel kind of polyketide-nonribosomal peptide hybrid product (PK-NRPs) that inhibits fatty acid biosynthesis in bacteria. Considering its great potential in biomedicine and biofarming, intensive studies have been conducted to increase the production of andrimid to overcome the excessive costs of chemosynthesis. In screening for species with broad-spectrum antibacterial activity, we detected andrimid in the fermentation products of Erwinia persicina BST187. To increase andrimid production, the BST187 fermentation medium formulation and fermentation conditions were optimized by using systematic design of experiments (One-Factor-At-A-Time, Plackett–Burman design, Response Surface Methodology).ResultsThe results indicate that the actual andrimid production reached 140.3 ± 1.28 mg/L under the optimized conditions (trisodium citrate dihydrate-30 g/L, beef extract-17.1 g/L, MgCl2·6H2O-100 mM, inoculation amount-1%, initial pH-7.0, fermentation time-36 h, temperature-19.7℃), which is 20-fold greater than the initial condition without optimization (7.00 ± 0.40 mg/L), consistent with the improved antibacterial effect of the fermentation supernatant.ConclusionsThe present study provides valuable information for improving andrimid production via optimization of the fermentation process, which will be of great value in the future industrialization of andrimid production.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

【 图 表 】

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