Cytotoxic effects of halophilic archaea metabolites on ovarian cancer cell lines

Microbial Cell Factories

Research

Krzysztof T. Krawczyk¹  Magdalena Kowalewicz-Kulbat¹  Camille Locht²  Magdalena Klink³  Izabela Szulc-Kielbik³  Sebastian Rykowski³  Marta Denel-Bobrowska³  Agnieszka B. Olejniczak³ 

[1] Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland;Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland;Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 – UMR9017 – CIIL – Center for Infection and Immunity of Lille, 59000, Lille, France;Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland;

关键词: Archaea;    Halophiles;    Ovarian cancer cells;    Cytotoxicity;   

DOI  :  10.1186/s12934-023-02206-y

 received in 2023-07-19, accepted in 2023-09-19,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundOvarian cancer is one of the most frequent and deadly gynaecological cancers, often resistant to platinum-based chemotherapy, the current standard of care. Halophilic microorganisms have been shown to produce a large variety of metabolites, some of which show toxicity to various cancer cell lines. However, none have yet been shown to be active against ovarian cancer cells. Here, we examined the effects of metabolites secreted by the halophilic archaea Halorhabdus rudnickae and Natrinema salaciae on various cancer cell lines, including ovarian cancer cell lines.Results1H NMR analyses of Hrd. rudnickae and Nnm. salaciae culture supernatants contain a complex mixture of metabolites that differ between species, and even between two different strains of the same species, such as Hrd. rudnickae strains 64T and 66. By using the MTT and the xCELLigence RTCA assays, we found that the secreted metabolites of all three halophilic strains expressed cytotoxicity to the ovarian cancer cell lines, especially A2780, as well as its cisplatin-resistant derivative A2780cis, in a dose-dependent manner. The other tested cell lines A549, HepG2, SK-OV-3 and HeLa were only minimally, or not at all affected by the archaeal metabolites, and this was only seen with the MTT assay.ConclusionsThe halophilic archaea Hrd. rudnickae and Nnm. salaciae, isolated from a Polish salt mine and Lake Medee in the Mediterranean Sea, respectively, secrete metabolites that are active against ovarian cancer cells, including those that are resistant to cisplatin. This opens potential new possibilities for the treatment of these frequent and deadly gynaecological cancers.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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