Genome-wide DNA methylation analysis of Astragalus and Danshen on the intervention of myofibroblast activation in idiopathic pulmonary fibrosis

BMC Pulmonary Medicine

Research

Yuan Hou¹  Xue Liu¹  Huaman Liu¹  Guoyu Wang²  Qingyin Liu³  Fan Wu³ 

[1] Affiliated Hospital of Shandong University of Traditional Chinese Medicine, No. 16369, Jing Shi Road, 250013, Jinan City, China;Capital Medical University, No. 10, Xizhang Road, Youanmenwai, Fengtai District, 100069, Beijing, China;Shandong University of Traditional Chinese Medicine, No. 4655, Daxue Road, University Science Park, Changqing District, 250355, Jinan City, China;

关键词: Idiopathic pulmonary fibrosis;    Danshen;    Astragalus;    DNA methylation;    Traditional chinese medicine;   

DOI  :  10.1186/s12890-023-02601-6

 received in 2023-03-30, accepted in 2023-08-09,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundIdiopathic pulmonary fibrosis (IPF), a chronic progressive interstitial lung disease of unknown etiology, is characterized by continuous damage to alveolar epithelial cells, abnormal repair of alveolar tissue, and alveolar wall scar formation. Currently, the recommended treatment for IPF in Western medicine is relatively limited. In contrast, traditional Chinese medicine and compound prescriptions show advantages in the diagnosis and treatment of IPF, which can be attributed to their multi-channel and multi-target characteristics and minimal side-effects. The purpose of this study was to further corroborate the effectiveness and significance of the traditional Chinese medications Astragalus and Danshen in IPF treatment.MethodsWe performed whole-genome methylation analysis on nine rat lung tissue samples to determine the epigenetic variation between IPF and non-fibrotic lungs using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses and quantitative reverse transcription polymerase chain reactions.ResultsWe identified differentially methylated regions and 105 associated key functional genes in samples related to IPF and Chinese medicine treatment. Based on the methylation levels and gene expression profiles between the Chinese medicine intervention and pulmonary fibrosis model groups, we speculated that Astragalus and Salvia miltiorrhiza (traditionally known as Danshen) act on the Isl1, forkhead box O3, and Sonic hedgehog genes via regulation at transcriptional and epigenetic levels during IPF.ConclusionsThese findings provide novel insights into the epigenetic regulation of IPF, indicate the effectiveness of Astragalus and Danshen in treating IPF, and suggest several promising therapeutic targets for preventing and treating IPF.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

【 图 表 】

Fig. 2

Fig. 6

Fig. 6

13731_2023_332_Article_IEq6.gif

13690_2023_1170_Article_IEq142.gif

Fig. 1