Gut microbiome profile of Chinese hypertension patients with and without type 2 diabetes mellitus

BMC Microbiology

Research

Jian Wu¹  Haoliang Zhou¹  Oushan Tang¹  Jin Lv¹  Hongying Ding¹  Yinhong Cheng¹  Shiye Dong²  Yue Xu²  Xiaosheng Hu³ 

[1] Department of Cardiovascular Medicine, Shaoxing Second hospital, Shaoxing, Zhejiang, China;Shanghai Biotecan Pharmaceuticals Co., Ltd, Shanghai, China;Shanghai Zhangjiang Institute of Medical Innovation, Shanghai, China;The First Affiliated Hospital of Medical College of Zhejiang University, Hangzhou, China;

关键词: Gut microbiota;    Hypertension;    Type 2 diabetes mellitus;    Comorbidity;    16S rRNA gene sequencing;   

DOI  :  10.1186/s12866-023-02967-x

 received in 2022-09-03, accepted in 2023-08-02,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundThe coexistence of hypertension and type 2 diabetes mellitus (T2DM) may largely increase the risk for cardiovascular disease. However, there is no clear consensus on the association between hypertension and the risk of diabetes. Gut microbiota plays important roles in the development of hypertension and T2DM, but whether there is difference between hypertension patients with or without T2DM has not been explored yet.MethodsWe recruited 101 hypertension patients in this study (72 patients without T2DM named HT group and 29 patients with T2DM named HT-T2DM group). Their blood samples were collected for testing clinical characteristics and fecal samples were tested for bacterial DNA using 16 S ribosomal RNA gene sequencing targeting the V3 and V4 region. The data of 40 samples were downloaded from project PRJNA815750 as health control (HC group) in this study. The community composition and structure of the microbiome, taxonomic difference, co-occurrence network and functional enrichment were analyzed by alpha/beta diversity, LEfSe, Fruchterman Reingold’s algorithm and PICRUSt2 functional analysis, respectively.ResultsAlpha and beta diversity analysis showed significant differences in microbial community richness and composition among the three groups. The HC group had a significantly higher Simpson index and a distinct microbiota community compared to the HT and HT-T2DM groups, as demonstrated by significant differences in unweighted and weighted UniFrac distances. The LEfSe analysis identified specific taxa that had significantly different abundance among the groups, such as Bacteroides uniformis, Blautia wexlerae, Alistipes putredinis, and Prevotella stercorea in the HC group, Prevotella copri and Phascolarctobacterium faecium in the HT group, and Klebsiella pneumoniae in the HT-T2DM group. Co-occurrence network analysis indicates that Prevotella copri, Mediterraneibacter gnavus, Alistipes onderdonkii and some unidentified species act as key nodes in the network. Differentially functional pathway identified by PICRUSt2 were concentrated in nutrition and energy metabolism, as well as the biosynthesis of other secondary metabolites.ConclusionsOur study found significant differences in microbial community richness, composition, and function among the healthy controls, hypertension patients with and without T2DM. Some specific taxa may explain this difference and serve as potential therapeutic targets for hypertension, T2DM, and their coexistence.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

【 图 表 】

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