Correlation between SDF-1α, CD34 positive hematopoietic stem cells and CXCR4 expression with liver fibrosis in CCl4 rat model

BMC Gastroenterology

Research

R.N Sherif¹  Kamal G Botros¹  Sara Abubakr¹  Adel Abdelmohdy Elhawary¹  Noha M. Hazem² 

[1] Human Anatomy & Embryology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt;Medical Biochemistry and Molecular Biology Department, Medical Experimental Research Center (MERC), Faculty of Medicine, Mansoura University, Algomhoria Street, 35516, Mansoura, Egypt;Pathological Sciences Department, Fakeeh College for Medical Sciences, Jeddah, Saudi Arabia;

关键词: SDF- 1α;    CD34;    CXCR4;    Hematopoietic stem cells;    Liver fibrosis;    Spontaneous reversion;   

DOI  :  10.1186/s12876-023-02932-y

 received in 2023-04-22, accepted in 2023-08-25,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundOne of the most frequent disorders is liver fibrosis. An improved understanding of the different events during the process of liver fibrosis & its reversibility could be helpful in its staging and in finding potential therapeutic agents.AimThe goal of this research was to evaluate the relationship among CD34 + HPSCs, SDF-1α, and CXCR4 receptor expression with the percentage of the area of hepatic fibrosis.Materials and methodsThirty-six male Sprague-Dawley rats were separated into the control group, liver injury group & spontaneous reversion group. The liver injury was induced by using 2 ml/kg CCl4 twice a week. Flow cytometric examination of CD34 + cells in the blood & liver was performed. Bone marrow & liver samples were taken for evaluation of the SDF-1α mRNA by PCR. Liver specimens were stained for histopathological and CXCR4 immuno-expression evaluation.ResultsIn the liver injury group, the hepatic enzymes, fibrosis area percentage, CXCR4 receptor expression in the liver, CD34 + cells in the blood and bone marrow & the level SDF-1α in the liver and its concentration gradient were statistically significantly elevated with the progression of the liver fibrosis. On the contrary, SDF-1α in the bone marrow was statistically significantly reduced with the development of liver fibrosis. During the spontaneous reversion group, all the studied parameters apart from SDF-1α in the bone marrow were statistically substantially decreased compared with the liver injury group. We found a statistically substantial positive correlation between fibrosis area and all of the following: liver enzymes, CXCR4 receptor expression in the liver, CD34 + cells in the blood and liver, and SDF- 1α in the liver and its concentration gradient. In conclusion, in CCl4 rat model, the fibrosis area is significantly correlated with many parameters in the blood, bone marrow, and liver, which can be used during the process of follow-up during the therapeutic interventions.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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