Molecular detection of Sodalis glossinidius, Spiroplasma species and Wolbachia endosymbionts in wild population of tsetse flies collected in Cameroon, Chad and Nigeria

BMC Microbiology

Research

Mohammed Nasir Shuaibu¹  Gloria Dada Chechet¹  Emmanuel Oluwadare Balogun¹  Mohammed Mamman²  Junaidu Kabir³  Soerge Kelm⁴  Mahamat Alhadj Moussa Ibrahim⁵  Djoukzoumka Signaboubo⁶  Youssouf Mouliom Mfopit⁷  Judith Sophie Engel⁸  Daniel Mbunkah Achukwi⁹ 

[1] Africa Centre of Excellence for Neglected Tropical Diseases and Forensic Biotechnology, Ahmadu Bello University, Zaria, Nigeria;Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria;Africa Centre of Excellence for Neglected Tropical Diseases and Forensic Biotechnology, Ahmadu Bello University, Zaria, Nigeria;Department of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria;Africa Centre of Excellence for Neglected Tropical Diseases and Forensic Biotechnology, Ahmadu Bello University, Zaria, Nigeria;Department of Veterinary Public Health and Preventive Medicine, Ahmadu Bello University, Zaria, Nigeria;Centre for Biomolecular Interactions Bremen, University of Bremen, Bremen, Germany;Department of Public Health, Faculty of Human Health Sciences, University of N’Djamena, N’Djamena, Chad;Institut de Recherche en Elevage pour le Développement, N’Djamena, Chad;Institute of Agricultural Research for Development, Yaounde, Cameroon;Africa Centre of Excellence for Neglected Tropical Diseases and Forensic Biotechnology, Ahmadu Bello University, Zaria, Nigeria;Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria;MARUM-PANGAEA, University of Bremen, Bremen, Germany;TOZARD Research Laboratory, P.O. Box 59, Bambili-Tubah, Bamenda, Cameroon;

关键词: Tsetse flies;    Symbionts;    Wolbachia;    Spiroplasma species;    Sodalis glossinidius;    Cameroon;    Chad;    Nigeria;   

DOI  :  10.1186/s12866-023-03005-6

 received in 2023-05-06, accepted in 2023-09-05,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundTsetse flies are cyclical vectors of African trypanosomiasis (AT). The flies have established symbiotic associations with different bacteria that influence certain aspects of their physiology. Vector competence of tsetse flies for different trypanosome species is highly variable and is suggested to be affected by bacterial endosymbionts amongst other factors. Symbiotic interactions may provide an avenue for AT control. The current study provided prevalence of three tsetse symbionts in Glossina species from Cameroon, Chad and Nigeria.ResultsTsetse flies were collected and dissected from five different locations. DNA was extracted and polymerase chain reaction used to detect presence of Sodalis glossinidius, Spiroplasma species and Wolbachia endosymbionts, using species specific primers. A total of 848 tsetse samples were analysed: Glossina morsitans submorsitans (47.52%), Glossina palpalis palpalis (37.26%), Glossina fuscipes fuscipes (9.08%) and Glossina tachinoides (6.13%). Only 95 (11.20%) were infected with at least one of the three symbionts. Among infected flies, six (6.31%) had Wolbachia and Spiroplasma mixed infection. The overall symbiont prevalence was 0.88, 3.66 and 11.00% respectively, for Sodalis glossinidius, Spiroplasma species and Wolbachia endosymbionts. Prevalence varied between countries and tsetse fly species. Neither Spiroplasma species nor S. glossinidius were detected in samples from Cameroon and Nigeria respectively.ConclusionThe present study revealed, for the first time, presence of Spiroplasma species infections in tsetse fly populations in Chad and Nigeria. These findings provide useful information on repertoire of bacterial flora of tsetse flies and incite more investigations to understand their implication in the vector competence of tsetse flies.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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Fig. 6