Effect of poly (lactic-co-glycolic acid) polymer nanoparticles loaded with vancomycin against Staphylococcus aureus biofilm

BMC Biotechnology

Research

Fereshte Kalhori¹  Mohammad-Ali Shahbazi²  Babak Asghari³  Ellahe Nouruzi³  Seyed Mostafa Hosseini⁴  Mohammad Reza Arabestani⁴  Reza Mahjoub⁵  Ehsan Nazarzadeh Zare⁶ 

[1] Biotechnology department, Hamadan University of Medical Sciences, Hamadan, IR, Iran;Department of Biomedical Engineering, University Medical Center Groningen, University of Groningen, Antonius Deusinglaan 1, 9713 AV, Groningen, The Netherlands;Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, IR, Iran;Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, IR, Iran;Infectious Disease Research Center, Hamadan University of Medical Sciences, Hamadan, IR, Iran;Department of Pharmacology and Toxicology, School of Pharmacy, Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences, Hamadan, Iran;School of Chemistry, Damghan University, Damghan, IR, Iran;

关键词: Staphylococcus aureus;    Vancomycin;    PLGA;    Lysostaphin;    Biofilm;   

DOI  :  10.1186/s12896-023-00811-8

 received in 2023-07-18, accepted in 2023-09-07,  发布年份 2023

来源: Springer

【 摘 要 】

Staphylococcus aureus is a unique challenge for the healthcare system because it can form biofilms, is resistant to the host's immune system, and is resistant to numerous antimicrobial therapies. The aim of this study was to investigate the effect of poly (lactic-co-glycolic acid) (PLGA) polymer nanoparticles loaded with vancomycin and conjugated with lysostaphin (PLGA-VAN-LYS) on inhibiting S. aureus biofilm formation. Nano drug carriers were produced using the double emulsion evaporation process. we examined the physicochemical characteristics of the nanoparticles, including particle size, polydispersity index (PDI), zeta potential, drug loading (DL), entrapment efficiency (EE), Lysostaphin conjugation efficiency (LCE), and shape. The effect of the nano drug carriers on S. aureus strains was evaluated by determining the minimum inhibitory concentration (MIC), conducting biofilm formation inhibition studies, and performing agar well diffusion tests. The average size, PDI, zeta potential, DL, EE, and LCE of PLGA-VAN-LYS were 320.5 ± 35 nm, 0.270 ± 0.012, -19.5 ± 1.3 mV, 16.75 ± 2.5%, 94.62 ± 2.6%, and 37% respectively. Both the agar well diffusion and MIC tests did not show a distinction between vancomycin and the nano drug carriers after 72 h. However, the results of the biofilm analysis demonstrated that the nano drug carrier had a stronger inhibitory effect on biofilm formation compared to the free drug. The use of this technology for treating hospital infections caused by the Staphylococcus bacteria may have favorable effects on staphylococcal infections, considering the efficacy of the nano medicine carrier developed in this study.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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