Quorum sensing gene regulation in Staphylococcus epidermidis reduces the attraction of Aedes aegypti (L.) (Diptera: Culicidae)

Frontiers in Microbiology

Microbiology

Heather R. Jordan¹  Jeffery K. Tomberlin²  Dongmin Kim²  Tawni L. Crippen³ 

[1] Department of Biological Sciences, Mississippi State University, Starkville, MS, United States;Department of Entomology, Texas A&M University, College Station, TX, United States;Southern Plains Agricultural Research Center, Agricultural Research Service, US Department of Agriculture, College Station, TX, United States;

关键词: microbes;    microbial volatile organic compounds;    mosquito attraction;    interkingdom communication;    gene regulation;   

DOI  :  10.3389/fmicb.2023.1208241

 received in 2023-04-18, accepted in 2023-05-22,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionIdentifying mechanisms regulating mosquito attraction to hosts is key to suppressing pathogen transmission. Historically, the ecology of the host microbial community and its influence on mosquito attraction, specifically, whether bacterial communication through quorum sensing (QS) modulates VOC production that affects mosquito behavior have not been extensively considered.MethodsBehavioral choice assays were applied along with volatile collection, followed by GC-MS and RNA transcriptome analyses of bacteria with and without a quorum-sensing inhibitor, furanone C-30.ResultsUtilizing the quorum-sensing inhibitor on a skin-inhabiting bacterium, Staphylococcus epidermidis, we disrupted its interkingdom communication with adult Aedes aegypti and mitigated their attraction to a blood-meal by 55.1%.DiscussionOne potential mechanism suppressing mosquito attraction could be the reduction (31.6% in our study) of bacterial volatiles and their associated concentrations by shifting S. epidermidis metabolic (12 of 29 up regulated genes) and stress (5 of 36 down regulated genes) responses. Manipulating the quorum-sensing pathways could serve as a mechanism to reduce mosquito attraction to a host. Such manipulations could be developed into novel control methods for pathogen-transmitting mosquitoes and other arthropods.

【 授权许可】

Unknown   
Copyright © 2023 Kim, Crippen, Jordan and Tomberlin.