The chemical profiling of Salvia plebeia during different growth periods and the biosynthesis of its main flavonoids ingredients

Frontiers in Plant Science

Plant Science

Minjian Qin¹  Yan Zhu¹  Ruirui Zhu¹  Yucheng Zhao¹  Shuai Li¹  Lanlan Sun¹  Ziyu Ye¹  Guoyong Xie¹  Yiqun Dai²  Hui Liu³ 

[1] Department of Resources Science of Traditional Chinese Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, China;Department of Resources Science of Traditional Chinese Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, China;School of Pharmacy, Bengbu Medical College, Bengbu, China;Yangzhou Center for Food and Drug Control, Yangzhou, China;

关键词: Salvia plebeia;    LC-MS/MS;    transcriptome;    flavonoid biosynthesis;    dynamic distribution;    harvest time;   

DOI  :  10.3389/fpls.2023.1228356

 received in 2023-05-24, accepted in 2023-07-24,  发布年份 2023

来源: Frontiers

【 摘 要 】

Salvia plebeia (Lamiaceae) is a valuable medicinal plant widely distributed across Asia and Oceania. However, the composition and accumulation patterns of its active ingredients in different organs during the growth and their biosynthetic mechanism remain unknown. Therefore, we conducted metabolite profiling, transcriptomic analysis, and biological functional verification to explore the distribution, accumulation, and biosynthesis mechanisms of flavonoids in S. plebeia. We identified 70 metabolites including 46 flavonoids, 16 phenolic acids, seven terpenoids, and one organic acid, of which 21 were previously unreported in S. plebeia. Combining metabolomic-transcriptomic analysis and biological functional verification, we identified the key genes involved in biosynthesis of its main active ingredients, hispidulin and homoplantaginin, including SpPAL, SpC4H, Sp4CL2, Sp4CL5, SpCHS1, SpCHI, SpFNS, SpF6H1, SpF6OMT1, SpF6OMT2, SpUGT1, SpUGT2, and SpUGT3. Using the identified genes, we reconstructed the hispidulin and homoplantaginin biosynthesis pathways in Escherichia coli, and obtained a yield of 5.33 and 3.86 mg/L for hispidulin and homoplantaginin, respectively. Our findings provide valuable insights into the changes in chemical components in different organs of S. plebeia during different growth and harvest stages and establishes a foundation for identifying and synthesizing its active components.

【 授权许可】

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Copyright © 2023 Dai, Ye, Liu, Zhu, Sun, Li, Xie, Zhu, Zhao and Qin