Bioactive volatile compounds from Penicillium digitatum-infected apples: Oviposition attractants for yellow peach moth Conogethes punctiferalis (Lepidoptera: Crambidae)

Frontiers in Sustainable Food Systems

Sustainable Food Systems

Peng Peng Ai¹  Zhe Yan¹  Min Zhao Zhang¹  Hong Gang Guo¹  Shuang Zhen Miao¹  Yan Li Du² 

[1] College of Bioscience and Resource Environment/Key Laboratory of Urban Agriculture (North China), Ministry of Agriculture and Rural Affairs of the People's Republic of China, Beijing University of Agriculture, Beijing, China;null;

关键词: Conogethes punctiferalis;    Penicillium digitatum;    plant-microbe-insect interactions;    chemical communication;    host plant volatile organic compounds;   

DOI  :  10.3389/fsufs.2023.1119717

 received in 2022-12-09, accepted in 2023-01-13,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionPlant-associated microbes critically shape the dynamics of plant-and insect-associated communities. In previous studies, we reported that the yellow peach moth Conogethes punctiferalis (YPM) preferred to Penicillium digitatum-infected apples (PDA) for oviposition. However, the underlying mechanisms remains unclear.MethodsIn the present study, the behavioral and physiological experiments were conducted to determine how P. digitatum affects the oviposition selection of mated YPM females via altering host plant volatile organic compounds (VOCs).ResultsMated YPM females were attracted to and laid more eggs on PDA than on non-infected apples (NIA), mechanically damaged apples (MDA), and P. digitatum in potato dextrose agar medium (PPD) in the oviposition selection experiments. Four-arm olfactometer assays further confirmed that odors in PDA were responsible for the attractiveness of mated YPM females. Further analyses showed that 38 VOCs were collected and identified from all treatments by GC-MS, with five specific VOCs (methyl 2-methylbutyrate, styrene, methyl caproate, butyl caprylate, and n-tetradecane) emitting from PDA. A principal component analysis (PCA) based on the absolute contents of 38 VOCs revealed a clear separation of PDA from NIA, MDA, and PPD. Moreover, when P. digitatum-induced specific VOCs were added to apples in individual or synthetic blends, there was a significantly higher percentage of mated YPM females to apples with individual or synthetic blends consisting of methyl 2-methylbutyrate, butyl caprylate, or n-tetradecane in Y-tube olfactometer experiments, suggesting that these three specific VOCs acted as predominant olfactory signals for mated YPM females to PDA.DiscussionTaken together, the microbe P. digitatum was an important driver of the interactions between YPMs and host plants by altering plant volatiles. These findings may form the basis for developing attractant baits for field trapping YPMs in the future.

【 授权许可】

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Copyright © 2023 Guo, Miao, Ai, Zhang, Yan and Du.