Development of a rat model of lymphedema and the implantation of a collagen-based medical device for therapeutic intervention

Frontiers in Cardiovascular Medicine

Cardiovascular Medicine

Ming-Huei Cheng¹  Ngan F. Huang²  Dimitrios Dionyssiou³  Anna T. Zhou⁴  Dung Nguyen⁴  Peter Deptula⁴  Maxim A. Moroz⁵  Stanley G. Rockson⁶  Gloria Sue⁷  Tatiana S. Zaitseva⁸  Peter Tabada⁸  Michael V. Paukshto⁸ 

[1] A+ Surgery Clinic, Taipei, Taiwan;Department of Cardiothoracic Surgery, Stanford University, Stanford, CA, United States;Stanford Cardiovascular Institute, Stanford University, Stanford, CA, United States;Center for Tissue Regeneration, Repair and Restoration, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, United States;Department of Chemical Engineering, Stanford University, Palo Alto, CA, United States, United States;Department of Plastic Surgery, Aristotle University of Thessaloniki, Greece;Department of Plastic and Reconstructive Surgery, Stanford University, Stanford, CA, United States;Department of Radiology, Stanford University, Stanford, CA, United States;Division of Cardiovascular Medicine, Stanford University, Stanford, CA, United States;Division of Plastic and Reconstructive Surgery, University of California San Francisco, San Francisco, CA, United States;Fibralign Corp, Union City, Thessaloniki, CA, United States;

关键词: lymphatic;    lymphedema;    scaffold;    rat;    animal model;    method;   

DOI  :  10.3389/fcvm.2023.1214116

 received in 2023-04-28, accepted in 2023-06-12,  发布年份 2023

来源: Frontiers

【 摘 要 】

Secondary lymphedema is a common condition among cancer survivors, and treatment strategies to prevent or treat lymphedema are in high demand. The development of novel strategies to diagnose or treat lymphedema would benefit from a robust experimental animal model of secondary lymphedema. The purpose of this methods paper is to describe and summarize our experience in developing and characterizing a rat hindlimb model of lymphedema. Here we describe a protocol to induce secondary lymphedema that takes advantage of micro computed tomography imaging for limb volume measurements and visualization of lymph drainage with near infrared imaging. To demonstrate the utility of this preclinical model for studying the therapeutic benefit of novel devices, we apply this animal model to test the efficacy of a biomaterials-based implantable medical device.

【 授权许可】

Unknown   
© 2023 Nguyen, Dionysiou, Zaitseva, Zhou, Sue, Deptula, Moroz, Tabada, Rockson, Paukshto, Cheng and Huang.