Automated Grooming Detection of Mouse by Three-Dimensional Convolutional Neural Network

Frontiers in Behavioral Neuroscience

Neuroscience

Masahito Yamamoto¹  Takahisa Murata²  Sakura Masuko²  Koji Kobayashi²  Naoaki Sakamoto²  Teruko Yamamoto² 

[1] Autonomous Systems Engineering Laboratory, Graduate School of Information Science and Technology, Hokkaido University, Sapporo, Japan;Department of Animal Radiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan;

关键词: grooming;    experimental animals;    automated detection;    mouse behavior;    deep learning;    convolutional neural network;    3D-CNN;   

DOI  :  10.3389/fnbeh.2022.797860

 received in 2021-10-20, accepted in 2022-01-03,  发布年份 2022

来源: Frontiers

【 摘 要 】

Grooming is a common behavior for animals to care for their fur, maintain hygiene, and regulate body temperature. Since various factors, including stressors and genetic mutations, affect grooming quantitatively and qualitatively, the assessment of grooming is important to understand the status of experimental animals. However, current grooming detection methods are time-consuming, laborious, and require specialized equipment. In addition, they generally cannot discriminate grooming microstructures such as face washing and body licking. In this study, we aimed to develop an automated grooming detection method that can distinguish facial grooming from body grooming by image analysis using artificial intelligence. Mouse behavior was recorded using a standard hand camera. We carefully observed videos and labeled each time point as facial grooming, body grooming, and not grooming. We constructed a three-dimensional convolutional neural network (3D-CNN) and trained it using the labeled images. Since the output of the trained 3D-CNN included unlikely short grooming bouts and interruptions, we set posterior filters to remove them. The performance of the trained 3D-CNN and filters was evaluated using a first-look dataset that was not used for training. The sensitivity of facial and body grooming detection reached 81.3% and 91.9%, respectively. The positive predictive rates of facial and body grooming detection were 83.5% and 88.5%, respectively. The number of grooming bouts predicted by our method was highly correlated with human observations (face: r = 0.93, body: r = 0.98). These results highlight that our method has sufficient ability to distinguish facial grooming and body grooming in mice.

【 授权许可】

Unknown   
Copyright © 2022 Sakamoto, Kobayashi, Yamamoto, Masuko, Yamamoto and Murata.