Advances in genetic manipulation of Chlamydia trachomatis

Frontiers in Immunology

Immunology

Dan Li¹  Danni Li²  Weiqiang Wan²  Jun Jiao² 

[1]Department of Respiratory Medicine, Center for Pathogen Biology and Infectious Diseases, Key Laboratory of Organ Regeneration and Transplantation of the Ministry of Education, The First Hospital of Jilin University, Changchun, China

[2]State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China

关键词: intracellular bacterium;    Chlamydia trachomatis;    genetic manipulation;    transformation;    challenges;   

DOI  :  10.3389/fimmu.2023.1209879

 received in 2023-04-21, accepted in 2023-06-14,  发布年份 2023

来源: Frontiers

【 摘 要 】

Chlamydia trachomatis, one species of Chlamydia spp., has the greatest impact on human health and is the main cause of bacterial sexually transmitted diseases and preventable blindness among all Chamydia spp. species. The obligate intracellular parasitism and unique biphasic developmental cycle of C. trachomatis are the main barriers for the development of tools of genetic manipulation. The past decade has witnessed significant gains in genetic manipulation of C. trachomatis, including chemical mutagenesis, group II intron-based targeted gene knockout, fluorescence-reported allelic exchange mutagenesis (FRAEM), CRISPR interference (CRISPRi) and the recently developed transposon mutagenesis. In this review, we discuss the current status of genetic manipulations of C. trachomatis and highlights new challenges in the nascent field of Chlamydia genetics.

【 授权许可】

Unknown   
Copyright © 2023 Wan, Li, Li and Jiao