Effect of the uronic acid composition of alginate in alginate/collagen hybrid hydrogel on chondrocyte behavior

Frontiers in Bioengineering and Biotechnology

Bioengineering and Biotechnology

Guoshuang Zheng¹  Fang Cao¹  Weiting Yu¹  Maoyuan Li¹  Chundong Xue¹  Minghui Hu¹  Hui Xie²  Dewei Zhao² 

[1] Laboratory of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, Dalian, China;National-Local Joint Engineering Laboratory for the Development of Orthopedic Implant Materials, Dalian, China;National-Local Joint Engineering Laboratory for the Development of Orthopedic Implant Materials, Dalian, China;Department of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, Dalian, China;

关键词: chondrocyte behavior;    alginate;    guluronate/mannuronate acid ratio (G/M ratio);    hydrogel;    mechanical properties;    cartilage tissue engineering;   

DOI  :  10.3389/fbioe.2023.1118975

 received in 2022-12-08, accepted in 2023-02-21,  发布年份 2023

来源: Frontiers

【 摘 要 】

Introduction: Developing a culture system that can effectively maintain chondrocyte phenotype and functionalization is a promising strategy for cartilage repair.Methods: An alginate/collagen (ALG/COL) hybrid hydrogel using different guluronate/mannuronate acid ratio (G/M ratio) of alginates (a G/M ratio of 64/36 and a G/M ratio of 34/66) with collagen was developed. The effects of G/M ratios on the properties of hydrogels and their effects on the chondrocytes behaviors were evaluated.Results: The results showed that the mechanical stiffness of the hydrogel was significantly affected by the G/M ratios of alginate. Chondrocytes cultured on Mid-G/M hydrogels exhibited better viability and phenotype preservation. Moreover, RT-qPCR analysis showed that the expression of cartilage-specific genes, including SOX9, COL2, and aggrecan was increased while the expression of RAC and ROCK1 was decreased in chondrocytes cultured on Mid-G/M hydrogels.Conclusion: These findings demonstrated that Mid-G/M hydrogels provided suitable matrix conditions for cultivating chondrocytes and may be useful in cartilage tissue engineering. More importantly, the results indicated the importance of taking alginate G/M ratios into account when designing alginate-based composite materials for cartilage tissue engineering.

【 授权许可】

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Copyright © 2023 Zheng, Xue, Cao, Hu, Li, Xie, Yu and Zhao.