Nematicidal effect of Beauveria species and the mycotoxin beauvericin against pinewood nematode Bursaphelenchus xylophilus

Frontiers in Forests and Global Change

Forests and Global Change

Baudilio Herrero¹  Steven J. Harte²  Matéo Bareyre³  Jorge Martín-García⁴  Jonathan Niño-Sánchez⁴  Tamara Sánchez-Gómez⁴  Julio Javier Díez⁴  Paula Zamora⁵ 

[1] Departamento de Ciencias Agroforestales, Universidad de Valladolid, Palencia, Spain;Natural Resources Institute, University of Greenwich, Kent, United Kingdom;Recognised Research Group AGROBIOTECH, UIC-370 (JCyL), Department of Plant Production and Forest Resources, University of Valladolid, Palencia, Spain;National School of Agricultural Engineering of Bordeaux (Bordeaux Sciences Agro), Gradignan, France;Recognised Research Group AGROBIOTECH, UIC-370 (JCyL), Department of Plant Production and Forest Resources, University of Valladolid, Palencia, Spain;Sustainable Forest Management Research Institute (iuFOR), University of Valladolid, Palencia, Spain;Recognised Research Group AGROBIOTECH, UIC-370 (JCyL), Department of Plant Production and Forest Resources, University of Valladolid, Palencia, Spain;Sustainable Forest Management Research Institute (iuFOR), University of Valladolid, Palencia, Spain;Sección Sanidad Forestal, Centro de Sanidad Forestal de Calabazanos, Consejería de Fomento y Medio Ambiente, Dirección General de Patrimonio Natural y Política Forestal, Palencia, Spain;

关键词: pine wilt disease;    integrated management;    biological control;    nematophagous fungi;    fungal toxins;   

DOI  :  10.3389/ffgc.2023.1229456

 received in 2023-05-26, accepted in 2023-08-11,  发布年份 2023

来源: Frontiers

【 摘 要 】

Introduction and main objectiveBursaphelenchus xylophilus, commonly known as pine wood nematode (PWN), is considered one of the greatest threats to European and Asian pines. Regarding its management, most efforts have been directed toward control measures for the major vector (Monochamus spp.) and screening for genetic resistance in its hosts. However, an integrated pest management strategy which also implements pinewood nematode control is currently lacking. The aim of this study was to evaluate the nematicidal effect of two Beauveria species, a genus well known for its entomopathogenic activity.Summary methodologyFor this purpose, in vitro antagonism tests of fungi (Beauveria bassiana and B. pseudobassiana) and the mycotoxin beauvericin (C45H57N3O9) on B. xylophilus populations were conducted. Finally, the production of beauvericin in B. bassiana and B. pseudobassiana strains was quantified by high-performance liquid chromatography - mass spectrometry (HPLC-MS).Results and discussionBoth the B. bassiana and B. pseudobassiana fungal species and the mycotoxin beauvericin showed a clear nematicidal effect on B. xylophilus populations, substantially reducing their survival rate and even attaining 100% mortality in one case. HPLC-MS analysis confirmed and quantified the production of beauvericin by B. bassiana and demonstrated for the first-time beauvericin production in B. pseudobassiana.Final conclusionThese findings highlight the potential of Beauveria species and the mycotoxin beauvericin to be implemented in an integrated pest management strategy to control both nematode and vector.

【 授权许可】

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Copyright © 2023 Sánchez-Gómez, Harte, Zamora, Bareyre, Díez, Herrero, Niño-Sánchez and Martín-García.