Upper respiratory tract mycobiome alterations in different kinds of pulmonary disease

Frontiers in Microbiology

Microbiology

Fangping Ding¹  Jing Wang¹  Ting Zhang²  Xingye Xu²  Ying Xue²  Jie Dong²  Fan Yang²  Qi Jin²  Xiangqi Hu²  Tao Liu² 

[1] Division of Pulmonary and Critical Care Medicine, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China;NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China;

关键词: airway mycobiome;    interstitial lung disease;    bacterial pneumonia;    fungal pneumonia;    asthma;    lung cancer;   

DOI  :  10.3389/fmicb.2023.1117779

 received in 2022-12-06, accepted in 2023-03-08,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionThe human respiratory tract is considered to be a polymicrobial niche, and an imbalance in the microorganism composition is normally associated with several respiratory diseases. In addition to the well-studied bacteriome, the existence of fungal species in the respiratory tract has drawn increasing attention and has been suggested to have a significant clinical impact. However, the understanding of the respiratory fungal microbiota (mycobiome) in pulmonary diseases is still insufficient.MethodsIn this study, we investigated the fungal community composition of oropharynx swab (OS) samples from patients with five kinds of pulmonary disease, including interstitial lung disease (ILD), bacterial pneumonia (BP), fungal pneumonia (FP), asthma (AS) and lung cancer (LC), and compared them with healthy controls (HCs), based on high-throughput sequencing of the amplified fungal internal transcribed spacer (ITS) region.ResultsThe results showed significant differences in fungal composition and abundance between disease groups and HCs. Malassezia was the most significant genus, which was much more abundant in pulmonary diseases than in the control. In addition, many common taxa were shared among different disease groups, but differences in taxa abundance and specific species in distinct disease groups were also observed. Based on linear discriminant analysis effect size (LefSe), each group had its characteristic species. Furthermore, some species showed a significant correlation with the patient clinical characteristics.DiscussionOur study deepened our understanding of the respiratory tract mycobiome in some diseases that are less studied and identified the commonalities and differences among different kinds of pulmonary disease. These results would provide the solid basis for further investigation of the association between the mycobiome and pathogenicity of pulmonary diseases.

【 授权许可】

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Copyright © 2023 Xu, Ding, Hu, Yang, Zhang, Dong, Xue, Liu, Wang and Jin.