Metabolic network reconstruction of Euglena gracilis: Current state, challenges, and applications

Frontiers in Microbiology

Microbiology

Chayakorn Pumas¹  Sahutchai Inwongwan²  Jeeraporn Pekkoh²  Pachara Sattayawat³ 

[1] Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand;Research Center in Bioresources for Agriculture, Industry and Medicine, Chiang Mai University, Chiang Mai, Thailand;Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand;Research Center of Microbial Diversity and Sustainable Utilizations, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand;Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand;Research Center of Microbial Diversity and Sustainable Utilizations, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand;Research Center in Bioresources for Agriculture, Industry and Medicine, Chiang Mai University, Chiang Mai, Thailand;

关键词: metabolic network reconstruction;    genome-scale metabolic model;    metabolic network modelling;    metabolic flux analysis;    Euglena gracilis;   

DOI  :  10.3389/fmicb.2023.1143770

 received in 2023-01-13, accepted in 2023-02-06,  发布年份 2023

来源: Frontiers

【 摘 要 】

A metabolic model, representing all biochemical reactions in a cell, is a prerequisite for several approaches in systems biology used to explore the metabolic phenotype of an organism. Despite the use of Euglena in diverse industrial applications and as a biological model, there is limited understanding of its metabolic network capacity. The unavailability of the completed genome data and the highly complex evolution of Euglena are significant obstacles to the reconstruction and analysis of its genome-scale metabolic model. In this mini-review, we discuss the current state and challenges of metabolic network reconstruction in Euglena gracilis. We have collated and present the available relevant data for the metabolic network reconstruction of E. gracilis, which could be used to improve the quality of the metabolic model of E. gracilis. Furthermore, we deliver the potential applications of the model in metabolic engineering. Altogether, it is supposed that this mini-review would facilitate the investigation of metabolic networks in Euglena and further lay out a direction for model-assisted metabolic engineering.

【 授权许可】

Unknown   
Copyright © 2023 Inwongwan, Pekkoh, Pumas and Sattayawat.