A Sec-dependent effector, CLIBASIA_04425, contributes to virulence in ‘Candidatus Liberibater asiaticus’

Frontiers in Plant Science

Plant Science

Changyong Zhou¹  Jun He¹  Shimin Fu¹  Xuefeng Wang¹  Shushe Zhang²  Song Zhang³  Tingchang Zhao⁴ 

[1]Citrus Research Institute, Southwest University/National Citrus Engineering Research Center, Chongqing, China

[2]Citrus Research Institute, Southwest University/National Citrus Engineering Research Center, Chongqing, China

[3]State Key Laboratory for Biology of Plant Diseases and Insect Pests, Chinese Academy of Agriculture Sciences, Institute of Plant Protection, Beijing, China

[4]Guangxi Citrus Breeding and Cultivation Engineering Technology Center Academy of Specialty Crops, Guangxi, Guilin, China

[5]State Key Laboratory for Biology of Plant Diseases and Insect Pests, Chinese Academy of Agriculture Sciences, Institute of Plant Protection, Beijing, China

关键词: Citrus Huanglongbing (HLB);    C;    effector;    C;    cell death;    virulence factor;   

DOI  :  10.3389/fpls.2023.1224736

 received in 2023-05-18, accepted in 2023-07-05,  发布年份 2023

来源: Frontiers

【 摘 要 】

Citrus Huanglongbing (HLB) is the most destructive citrus disease worldwide, mainly caused by ‘Candidatus Liberibacter asiaticus’ (CLas). It encodes a large number of Sec-dependent effectors that contribute to HLB progression. In this study, an elicitor triggering ROS burst and cell death in Nicotiana benthamiana, CLIBASIA_04425 (CLas4425), was identified. Of particular interest, its cell death-inducing activity is associated with its subcellular localization and the cytoplasmic receptor Botrytis-induced kinase 1 (BIK1). Compared with CLas infected psyllids, CLas4425 showed higher expression level in planta. The transient expression of CLas4425 in N. benthamiana and its overexpression in Citrus sinensis enhanced plant susceptibility to Pseudomonas syringae pv. tomato DC3000 ΔhopQ1-1 and CLas, respectively. Furthermore, the salicylic acid (SA) level along with the expression of genes NPR1/EDS1/NDR1/PRs in SA signal transduction was repressed in CLas4425 transgenic citrus plants. Taken together, CLas4425 is a virulence factor that promotes CLas proliferation, likely by interfering with SA-mediated plant immunity. The results obtained facilitate our understanding of CLas pathogenesis.

【 授权许可】

Unknown   
Copyright © 2023 Zhang, Wang, He, Zhang, Zhao, Fu and Zhou