Application of CRISPR-Cas system in the diagnosis and therapy of ESKAPE infections

Frontiers in Cellular and Infection Microbiology

Cellular and Infection Microbiology

Zhiqin Ying¹  Yizheng Qian¹  Yongxiang Zhao¹  Huanhuan Liu¹  Li Yang¹  Min Li¹  Dapeng Zhou²  Guangtao Huang³ 

[1] Department of Burns and Plastic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, China;The Collaborative Innovation Center of Tissue Damage Repair and Regeneration Medicine of Zunyi Medical University, Zunyi, China;Department of Burns and Plastic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, China;The Collaborative Innovation Center of Tissue Damage Repair and Regeneration Medicine of Zunyi Medical University, Zunyi, China;Department of Burn Plastic and Wound Repair Surgery, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China;Department of Burns and Plastic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, China;The Collaborative Innovation Center of Tissue Damage Repair and Regeneration Medicine of Zunyi Medical University, Zunyi, China;Department of Burn and Plastic Surgery, Department of Wound Repair, Shenzhen Institute of Translational Medicine, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, China;

关键词: CRISPR-Cas;    ESKAPE;    pathogen;    infection;    diagnosis;    therapy;   

DOI  :  10.3389/fcimb.2023.1223696

 received in 2023-05-16, accepted in 2023-07-24,  发布年份 2023

来源: Frontiers

【 摘 要 】

Antimicrobial-resistant ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species) pathogens represent a global threat to human health. ESKAPE pathogens are the most common opportunistic pathogens in nosocomial infections, and a considerable number of their clinical isolates are not susceptible to conventional antimicrobial therapy. Therefore, innovative therapeutic strategies that can effectively deal with ESKAPE pathogens will bring huge social and economic benefits and ease the suffering of tens of thousands of patients. Among these strategies, CRISPR (clustered regularly interspaced short palindromic repeats) system has received extra attention due to its high specificity. Regrettably, there is currently no direct CRISPR-system-based anti-infective treatment. This paper reviews the applications of CRISPR-Cas system in the study of ESKAPE pathogens, aiming to provide directions for the research of ideal new drugs and provide a reference for solving a series of problems caused by multidrug-resistant bacteria (MDR) in the post-antibiotic era. However, most research is still far from clinical application.

【 授权许可】

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Copyright © 2023 Qian, Zhou, Li, Zhao, Liu, Yang, Ying and Huang