Combination of violet light irradiation and collagenase treatments in a rabbit model of keratoconus

Frontiers in Medicine

Medicine

Hidenaga Kobashi¹  Kazuo Tsubota¹  Takashi Yano² 

[1] Department of Ophthalmology, School of Medicine, Keio University, Tokyo, Japan;Tsubota Laboratory Inc., Tokyo, Japan;Life Science Laboratories, Ltd., Osaka, Japan;

关键词: keratoconus;    animal model;    collagenase;    violet light;    corneal cross-linking;   

DOI  :  10.3389/fmed.2023.1109689

 received in 2022-11-28, accepted in 2023-04-28,  发布年份 2023

来源: Frontiers

【 摘 要 】

PurposeWe evaluated the use of collagenase treatment to generate a rabbit model of keratoconus and the impact of violet light (VL) irradiation on the disease model in six Japanese White rabbits.MethodsAfter epithelial debridement, the collagenase group was treated with a collagenase type II solution for 30 min; the control group was treated with a solution without collagenase. Three rabbits also underwent VL irradiation (375 nm, irradiance 310 μW/cm2) for 3 h daily for 7 days after topical collagenase application. Slit-lamp microscopy results, steep keratometry (Ks), corneal astigmatism, central corneal thickness, and axial length were examined before and after the procedure. The corneas were obtained on day 7 for biomechanical evaluation.ResultsA significant increase in Ks and corneal astigmatism was observed in the collagenase and VL irradiation groups compared with the control group on day 7. No significant difference was found in the change in corneal thickness between the groups. The elastic modulus at 3, 5, and 10% strain was significantly lower in the collagenase group than in the control group. There was no significant difference in the elastic modulus at any level of strain between the collagenase and VL irradiation groups. The average axial length at day 7 was significantly longer in the collagenase and VL irradiation groups than in the control group. Collagenase treatment induced a model of keratoconus by steepening the keratometric and astigmatic values. There was no significant difference in the observed elastic behavior of normal and ectatic corneas under physiologically relevant stress levels.ConclusionVL irradiation did not cause regression of corneal steepening in a collagenase-induced model during short-term observation.

【 授权许可】

Unknown   
Copyright © 2023 Kobashi, Yano and Tsubota.