Genome-wide association study in two-row spring barley landraces identifies QTL associated with plantlets root system architecture traits in well-watered and osmotic stress conditions

Frontiers in Plant Science

Plant Science

Alexie Techer¹  Véronique Bergougnoux¹  Cintia F. Marchetti¹  Thu D. Nguyen¹  Mortaza Khodaeiaminjan¹  Nikola Kořínková¹  Pascal Gantet²  Andreas Graner³  Jianting Chu⁴  Kerstin Neumann⁵  Dominic Knoch⁵  Ingrid Doridant⁶  Valentin Bertholomey⁶  Marie Rose Ndella Thiaw⁷ 

[1] Czech Advanced Technology and Research Institute, Palacký University in Olomouc, Olomouc, Czechia;Czech Advanced Technology and Research Institute, Palacký University in Olomouc, Olomouc, Czechia;Unité Mixte de Recherche DIADE, Université de Montpellier, IRD, CIRAD, Montpellier, France;Department Genebank, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany;Department of Breeding Research, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany;Department of Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany;Limagrain Field Seeds, Traits and Technologies, Groupe Limagrain Centre de Recherche, Chappes, France;Unité Mixte de Recherche DIADE, Université de Montpellier, IRD, CIRAD, Montpellier, France;

关键词: barley landraces;    osmotic stress;    root system architecture;    GWAS;    QTL;    candidate gene;   

DOI  :  10.3389/fpls.2023.1125672

 received in 2022-12-16, accepted in 2023-03-15,  发布年份 2023

来源: Frontiers

【 摘 要 】

Water availability is undoubtedly one of the most important environmental factors affecting crop production. Drought causes a gradual deprivation of water in the soil from top to deep layers and can occur at diverse stages of plant development. Roots are the first organs that perceive water deficit in soil and their adaptive development contributes to drought adaptation. Domestication has contributed to a bottleneck in genetic diversity. Wild species or landraces represent a pool of genetic diversity that has not been exploited yet in breeding program. In this study, we used a collection of 230 two-row spring barley landraces to detect phenotypic variation in root system plasticity in response to drought and to identify new quantitative trait loci (QTL) involved in root system architecture under diverse growth conditions. For this purpose, young seedlings grown for 21 days in pouches under control and osmotic-stress conditions were phenotyped and genotyped using the barley 50k iSelect SNP array, and genome-wide association studies (GWAS) were conducted using three different GWAS methods (MLM GAPIT, FarmCPU, and BLINK) to detect genotype/phenotype associations. In total, 276 significant marker-trait associations (MTAs; p-value (FDR)< 0.05) were identified for root (14 and 12 traits under osmotic-stress and control conditions, respectively) and for three shoot traits under both conditions. In total, 52 QTL (multi-trait or identified by at least two different GWAS approaches) were investigated to identify genes representing promising candidates with a role in root development and adaptation to drought stress.

【 授权许可】

Unknown   
Copyright © 2023 Khodaeiaminjan, Knoch, Ndella Thiaw, Marchetti, Kořínková, Techer, Nguyen, Chu, Bertholomey, Doridant, Gantet, Graner, Neumann and Bergougnoux