Genome-wide identification, expression pattern and genetic variation analysis of SWEET gene family in barley reveal the artificial selection of HvSWEET1a during domestication and improvement

Frontiers in Plant Science

Plant Science

Lei Liu¹  Wenhao Yue¹  Kangfeng Cai¹  Junmei Wang¹  Xue Xia² 

[1] Key Laboratory of Digital Dry Land Crops of Zhejiang Province, Zhejiang Academy of Agricultural Sciences, Hangzhou, China;National Barley Improvement Center, Hangzhou, China;Key Laboratory of Digital Dry Land Crops of Zhejiang Province, Zhejiang Academy of Agricultural Sciences, Hangzhou, China;National Barley Improvement Center, Hangzhou, China;College of Advanced Agricultural Sciences, Zhejiang Agricultural and Forestry University, Hangzhou, China;

关键词: barley;    SWEET;    expression profile;    haplotype;    domestication;   

DOI  :  10.3389/fpls.2023.1137434

 received in 2023-01-04, accepted in 2023-02-02,  发布年份 2023

来源: Frontiers

【 摘 要 】

SWEET (Sugars Will Eventually be Exported Transporter) proteins, an essential class of sugar transporters, are involved in vital biological processes of plant growth and development. To date, systematical analysis of SWEET family in barley (Hordeum vulgare) has not been reported. In this study, we genome-wide identified 23 HvSWEET genes in barley, which were further clustered into four clades by phylogenetic tree. The members belonging to the same clade showed relatively similar gene structures and conserved protein motifs. Synteny analysis confirmed the tandem and segmental duplications among HvSWEET genes during evolution. Expression profile analysis demonstrated that the patterns of HvSWEET genes varied and the gene neofunctionalization occurred after duplications. Yeast complementary assay and subcellular localization in tobacco leaves suggested that HvSWEET1a and HvSWEET4, highly expressed in seed aleurone and scutellum during germination, respectively, functioned as plasma membrane hexose sugar transporters. Furthermore, genetic variation detection indicated that HvSWEET1a was under artificial selection pressure during barley domestication and improvement. The obtained results facilitate our comprehensive understanding and further functional investigations of barley HvSWEET gene family, and also provide a potential candidate gene for de novo domestication breeding of barley.

【 授权许可】

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Copyright © 2023 Yue, Cai, Xia, Liu and Wang