Antioxidant and antibacterial activity of Apis laboriosa honey against Salmonella enterica serovar Typhimurium

Frontiers in Nutrition

Nutrition

Qingya Zhang¹  Wenrong Wu¹  Xiaoqing Miao²  Yuanyuan Tian³  Weihua Tan⁴  Wenchao Yang⁵  Siwei Miao⁶  Haiou Kuang⁷ 

[1] Bee Product Processing and Application Research Center of the Ministry of Education, Fuzhou, Fujian, China;College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China;Bee Product Processing and Application Research Center of the Ministry of Education, Fuzhou, Fujian, China;M.X.’s Expert Workstation, Pu’er, Yunnan, China;College of Animal Science (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou, Fujian, China;Bee Product Processing and Application Research Center of the Ministry of Education, Fuzhou, Fujian, China;College of Animal Science (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou, Fujian, China;Bee Product Processing and Application Research Center of the Ministry of Education, Fuzhou, Fujian, China;College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China;College of Animal Science (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou, Fujian, China;Bee Product Processing and Application Research Center of the Ministry of Education, Fuzhou, Fujian, China;College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China;M.X.’s Expert Workstation, Pu’er, Yunnan, China;M.X.’s Expert Workstation, Pu’er, Yunnan, China;M.X.’s Expert Workstation, Pu’er, Yunnan, China;Research Institute of Eastern Honeybee, Yunnan Agricultural University, Kunming, Yunnan, China;

关键词: Apis laboriosa;    antioxidant activity;    antibacterial activity;    Salmonella;    proteomics;   

DOI  :  10.3389/fnut.2023.1181492

 received in 2023-03-07, accepted in 2023-04-18,  发布年份 2023

来源: Frontiers

【 摘 要 】

Salmonella enterica serovar Typhimurium (S. Typhimurium) is a common food-borne pathogen that commonly causes gastroenteritis in humans and animals. Apis laboriosa honey (ALH) harvested in China has significant antibacterial activity against Staphylococcus aureus, Escherichia coli, and Bacillus subtilis. We hypothesize that ALH has antibacterial activity against S. Typhimurium. The physicochemical parameters, minimum inhibitory and bactericidal concentrations (MIC and MBC) and the possible mechanism were determined. The results showed that there were significantly different physicochemical parameters, including 73 phenolic compounds, among ALH samples harvested at different times and from different regions. Their antioxidant activity was affected by their components, especially total phenol and flavonoid contents (TPC, TFC), which had a high correlation with antioxidant activities except for the O2- assay. The MIC and MBC of ALH against S. Typhimurium were 20–30% and 25–40%, respectively, which were close to those of UMF5+ manuka honey. The proteomic experiment revealed the possible antibacterial mechanism of ALH1 at IC50 (2.97%, w/v), whose antioxidant activity reduced the bacterial reduction reaction and energy supply, mainly by inhibiting the citrate cycle (TCA cycle), amino acid metabolism pathways and enhancing the glycolysis pathway. The results provide a theoretical basis for the development of bacteriostatic agents and application of ALH.

【 授权许可】

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Copyright © 2023 Tan, Tian, Zhang, Miao, Wu, Miao, Kuang and Yang.