Maternal high-fat diet decreases milk endocannabinoids with sex-specific changes in the cannabinoid and dopamine signaling and food preference in rat offspring

Frontiers in Endocrinology

Endocrinology

Georgia C. Atella¹  Henrique M. G. Pereira²  Gustavo R. C. Santos²  Julia C. B. Costa³  Larissa B. Fassarella³  Carmen C. Pazos-Moura³  Mariana M. Almeida³  Camilla P. Dias-Rocha³  Yamara S. Oliveira³  Isis H. Trevenzoli³  Juliana Woyames³ 

[1] Laboratório de Bioquímica de Lipídios e Lipoproteínas, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil;Laboratório de Desenvolvimento Tecnológico, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil;Laboratório de Endocrinologia Molecular, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil;

关键词: maternal high-fat diet;    metabolic programming;    breast milk;    endocannabinoid system;    obesity;    rat development;    food preference;    dopamine system;   

DOI  :  10.3389/fendo.2023.1087999

 received in 2022-11-02, accepted in 2023-02-08,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionMaternal high-fat (HF) diet during gestation and lactation programs obesity in rat offspring associated with sex-dependent and tissue-specific changes of the endocannabinoid system (ECS). The ECS activation induces food intake and preference for fat as well as lipogenesis. We hypothesized that maternal HF diet would increase the lipid endocannabinoid levels in breast milk programming cannabinoid and dopamine signaling and food preference in rat offspring.MethodsFemale Wistar rats were assigned into two experimental groups: control group (C), which received a standard diet (10% fat), or HF group, which received a high-fat diet (29% fat) for 8 weeks before mating and during gestation and lactation. Milk samples were collected to measure endocannabinoids and fatty acids by mass spectrometry. Cannabinoid and dopamine signaling were evaluated in the nucleus accumbens (NAc) of male and female weanling offspring. C and HF offspring received C diet after weaning and food preference was assessed in adolescence.ResultsMaternal HF diet reduced the milk content of anandamide (AEA) (p<0.05) and 2-arachidonoylglycerol (2-AG) (p<0.05). In parallel, maternal HF diet increased adiposity in male (p<0.05) and female offspring (p<0.05) at weaning. Maternal HF diet increased cannabinoid and dopamine signaling in the NAc only in male offspring (p<0.05), which was associated with higher preference for fat in adolescence (p<0.05).ConclusionContrary to our hypothesis, maternal HF diet reduced AEA and 2-AG in breast milk. We speculate that decreased endocannabinoid exposure during lactation may induce sex-dependent adaptive changes of the cannabinoid-dopamine crosstalk signaling in the developing NAc, contributing to alterations in neurodevelopment and programming of preference for fat in adolescent male offspring.

【 授权许可】

Unknown   
Copyright © 2023 Dias-Rocha, Costa, Oliveira, Fassarella, Woyames, Atella, Santos, Pereira, Pazos-Moura, Almeida and Trevenzoli