期刊论文详细信息
Frontiers in Cardiovascular Medicine | |
Fibrin deposition on bovine pericardium tissue used for bioprosthetic heart valve drives its calcification | |
Cardiovascular Medicine | |
Julie Piquet1  Jeanne Rancic2  Ulysse Richez2  David M. Smadja3  Salma El Batti4  Bastien Poitier4  | |
[1]Biosurgical Research Lab (Carpentier Foundation, Université de Paris Cité and AP-HP), Paris, France | |
[2]Université de Paris Cité, Innovative Therapies in Haemostasis, INSERM UMR-S1140, Paris, France | |
[3]Université de Paris Cité, Innovative Therapies in Haemostasis, INSERM UMR-S1140, Paris, France | |
[4]Biosurgical Research Lab (Carpentier Foundation, Université de Paris Cité and AP-HP), Paris, France | |
[5]Hematology Department, AP-HP, Georges Pompidou European Hospital, Paris, France | |
[6]Université de Paris Cité, Innovative Therapies in Haemostasis, INSERM UMR-S1140, Paris, France | |
[7]Cardiovascular Surgery Department, AP-HP, Georges Pompidou European Hospital, Paris, France | |
[8]Biosurgical Research Lab (Carpentier Foundation, Université de Paris Cité and AP-HP), Paris, France | |
关键词: bioprosthetic heart valve; pericardium tissue; thrombosis; fibrin; calcification; | |
DOI : 10.3389/fcvm.2023.1198020 | |
received in 2023-04-03, accepted in 2023-07-13, 发布年份 2023 | |
来源: Frontiers | |
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【 摘 要 】
BackgroundBioprosthetic heart valves (BHVs) are less thrombogenic than mechanical prostheses; however, BHV thrombosis has been proposed as a risk factor for premature BHV degeneration.ObjectivesWe aimed to explore whether fibrin deposition on bovine pericardium tissue could lead to calcification.MethodFibrin clot was obtained by blending three reagents, namely, CRYOcheck™ Pooled Normal Plasma (4/6), tissue factor + phospholipids (Thrombinoscope BV), and 100 mM calcium (1/6), and deposited on pericardium discs. Non-treated and fibrin-treated bovine pericardium discs were inserted into the subcutaneous tissue of 12-day-old Wistar rats and sequentially explanted on days 5, 10, and 15. Calcium content was measured with acetylene flame atomic absorption spectrophotometry. Histological analysis was performed using hematoxylin–eosin staining, Von Kossa staining, and immunohistochemistry.ResultsCalcification levels were significantly higher in fibrin-treated bovine pericardium discs compared to those in non-treated bovine pericardium discs (27.45 ± 23.05 µg/mg vs. 6.34 ± 6.03 µg/mg on day 5, 64.34 ± 27.12 µg/mg vs. 34.21 ± 19.11 µg/mg on day 10, and 64.34 ± 27.12 µg/mg vs. 35.65 ± 17.84 µg/mg on day 15; p < 0.001). Von Kossa staining confirmed this finding. In hematoxylin–eosin staining, the bovine pericardium discs were more extensively and deeply colonized by inflammatory-like cells, particularly T lymphocytes (CD3+ cells), when pretreated with fibrin.ConclusionFibrin deposition on bovine pericardium tissue treated with glutaraldehyde, used for BHV, led to increased calcification in a rat model. BHV thrombosis could be one of the triggers for calcification and BHV deterioration.【 授权许可】
Unknown
© 2023 Poitier, Rancic, Richez, Piquet, El Batti and Smadja.
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