Host A-to-I RNA editing signatures in intracellular bacterial and single-strand RNA viral infections

Frontiers in Immunology

Immunology

Yan-Shan Liu¹  Shou-Yue Xia²  Jian-Huan Chen²  Jia-Huan Li²  Zhi-Xin Wang²  Di Gao²  Yu-Ning Li²  Yun-Yun Jin²  Zhi-Yuan Wei²  Xu-Bin Pan²  Yan-Shuo Wen² 

[1] Department of Pediatric Laboratory, Wuxi Children’s Hospital, Wuxi, Jiangsu, China;Laboratory of Genomic and Precision Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu, China;Joint Primate Research Center for Chronic Diseases, Institute of Zoology of Guangdong Academy of Science, Jiangnan University, Wuxi, Jiangsu, China;Jiangnan University Brain Institute, Wuxi, Jiangsu, China;

关键词: A-to-I RNA editing;    bacterial infection;    viral infection;    intracellular bacterial pathogens;    pneumonia;   

DOI  :  10.3389/fimmu.2023.1121096

 received in 2022-12-13, accepted in 2023-03-13,  发布年份 2023

来源: Frontiers

【 摘 要 】

BackgroundMicrobial infection is accompanied by remodeling of the host transcriptome. Involvement of A-to-I RNA editing has been reported during viral infection but remains to be elucidated during intracellular bacterial infections.ResultsHerein we analyzed A-to-I RNA editing during intracellular bacterial infections based on 18 RNA-Seq datasets of 210 mouse samples involving 7 tissue types and 8 intracellular bacterial pathogens (IBPs), and identified a consensus signature of RNA editing for IBP infections, mainly involving neutrophil-mediated innate immunity and lipid metabolism. Further comparison of host RNA editing patterns revealed remarkable similarities between pneumonia caused by IBPs and single-strand RNA (ssRNA) viruses, such as altered editing enzyme expression, editing site numbers, and levels. In addition, functional enrichment analysis of genes with RNA editing highlighted that the Rab GTPase family played a common and vital role in the host immune response to IBP and ssRNA viral infections, which was indicated by the consistent up-regulated RNA editing of Ras-related protein Rab27a. Nevertheless, dramatic differences between IBP and viral infections were also observed, and clearly distinguished the two types of intracellular infections.ConclusionOur study showed transcriptome-wide host A-to-I RNA editing alteration during IBP and ssRNA viral infections. By identifying and comparing consensus signatures of host A-to-I RNA editing, our analysis implicates the importance of host A-to-I RNA editing during these infections and provides new insights into the diagnosis and treatment of infectious diseases.

【 授权许可】

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Copyright © 2023 Wei, Wang, Li, Wen, Gao, Xia, Li, Pan, Liu, Jin and Chen