Identification and functional analysis of ZmDLS associated with the response to biotic stress in maize

Frontiers in Plant Science

Plant Science

Lina Xu¹  Honghong Fan²  Yiting Wang²  Jiali Li²  Jie Zou²  Fanna Kong³  Jiaxin Li³  Dafeng Xu³  Tingchun Li³  Lin Zhang³  Huaying Yang³ 

[1] Institute of Plant Protection and Agro-products Safety, Anhui Academy of Agricultural Sciences, Hefei, China;School of Life Science, Anhui Agricultural University, Hefei, China;Tobacco Research Institute, Anhui Academy of Agricultural Sciences, Hefei, China;

关键词: maize;    terpene synthase;    corn borer bite;    Fusarium graminearum;    defense response;    transcription factor;   

DOI  :  10.3389/fpls.2023.1162826

 received in 2023-02-10, accepted in 2023-06-26,  发布年份 2023

来源: Frontiers

【 摘 要 】

Terpenes are the main class of secondary metabolites produced in response to pest and germ attacks. In maize (Zea mays L.), they are the essential components of the herbivore-induced plant volatile mixture, which functioned as a direct or indirect defense against pest and germ attacks. In this study, 43 maize terpene synthase gene (ZmTPS) family members were systematically identified and analyzed through the whole genomes of maize. Nine genes, including Zm00001d032230, Zm00001d045054, Zm00001d024486, Zm00001d004279, Zm00001d002351, Zm00001d002350, Zm00001d053916, Zm00001d015053, and Zm00001d015054, were isolated for their differential expression pattern in leaves after corn borer (Ostrinia nubilalis) bite. Additionally, six genes (Zm00001d045054, Zm00001d024486, Zm00001d002351, Zm00001d002350, Zm00001d015053, and Zm00001d015054) were significantly upregulated in response to corn borer bite. Among them, Zm00001d045054 was cloned. Heterologous expression and enzyme activity assays revealed that Zm00001d045054 functioned as d-limonene synthase. It was renamed ZmDLS. Further analysis demonstrated that its expression was upregulated in response to corn borer bites and Fusarium graminearum attacks. The mutant of ZmDLS downregulated the expressions of Zm00001d024486, Zm00001d002351, Zm00001d002350, Zm00001d015053, and Zm00001d015054. It was more attractive to corn borer bites and more susceptible to F. graminearum infection. The yeast one-hybrid assay and dual-luciferase assay showed that ZmMYB76 and ZmMYB101 could upregulate the expression of ZmDLS by binding to the promoter region. This study may provide a theoretical basis for the functional analysis and transcriptional regulation of terpene synthase genes in crops.

【 授权许可】

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Copyright © 2023 Wang, Zou, Li, Kong, Xu, Xu, Li, Yang, Zhang, Li and Fan