Association of Human Leukocyte Antigen (HLA) class II (DRB1 and DQB1) alleles and haplotypes with Rheumatoid Arthritis in Sudanese patients

Frontiers in Immunology

Immunology

Mohammed Salman Akhtar¹  Adil Ahmed Ali²  Khalid Eltahir Khalid³  Osman Khalafalla Saeed⁴  Somaya Elhaj Mohammed⁵ 

[1] Department of Basic Medical Sciences, Faculty of Applied Medical Sciences, Al-Baha University, Al-Baha, Saudi Arabia;Department of Biochemistry and Nutrition, Faculty of Medicine, University of Gezira, Wad Medani, Sudan;Department of Biochemistry and Nutrition, Faculty of Medicine, University of Gezira, Wad Medani, Sudan;Department of Basic Medical Sciences, Faculty of Applied Medical Sciences, Al-Baha University, Al-Baha, Saudi Arabia;Department of Internal Medicine, Faculty of Medicine, University of Gezira, Wad Medani, Sudan;Department of Pathology, Faculty of Medicine, University of Gezira, Wad Medani, Sudan;

关键词: rheumatoid arthritis;    HLA-DRB1;    HLA-DQB1;    anti-CCP;    rheumatoid factor;   

DOI  :  10.3389/fimmu.2023.1178546

 received in 2023-03-02, accepted in 2023-06-05,  发布年份 2023

来源: Frontiers

【 摘 要 】

The aim of this study was to determine the Human Leukocyte Antigen (HLA) class II (DRB1 and DQB1) alleles and haplotype frequency in Rheumatoid Arthritis (RA) in the Sudanese population. The frequency of HLA-DRB1 and -DQB1 alleles and DRB1-DQB1 haplotypes were determined in 122 RA patients and 100 controls. HLA alleles were genotyped by the polymerase chain reaction-sequence specific primers (PCR-SSP) method. In RA patients, HLA-DRB1*04 and *10 alleles were high in frequency (9.6% vs 14.2%, P = 0.038 and P = 0.042, respectively), and dependently on anti-citrullinated protein antibodies (ACPAs) seropositivity (P = 0.044 and P = 0.027, respectively). In contrast, the frequency of the HLA-DRB1*07 allele was significantly low in patients than in controls (11.7% vs 5.0%, P = 0.010). Moreover, the HLA-DQB1*03 allele was strongly associated with RA risk (42.2%, P = 2.2x10-8), whereas, HLA-DQB1*02 and *06 showed protective effects against RA (23.1% and 42.2%, P = 0.024 and P = 2.2x10-6, respectively). Five different HLA haplotypes, DRB1*03-DQB1*03 (P = 0.00003), DRB1*04-DQB1*03 (P = 0.00014), DRB1*08-DQB1*03 (P = 0.027), DRB1*13-DQB1*02 (P = 0.004), and DRB1*13-DQB1*03 (P = 3.79x10-8) were significantly associated with RA risk, while 3 protective haplotypes, DRB1*03-DQB1*02 (Pc = 0.008), DRB1*07-DQB1*02 (Pc = 0.004), and DRB1*13-DQB1*06 (Pc = 0.02) were identified. This is the first study determining the association between HLA class II alleles and haplotypes and RA risk in our population.

【 授权许可】

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Copyright © 2023 Ali, Khalid, Mohammed, Akhtar and Saeed