Marker-assisted introgression of wild chromosome segments conferring resistance to fungal foliar diseases into peanut (Arachis hypogaea L.)

Frontiers in Plant Science

Plant Science

João Francisco dos Santos¹  Ignácio José Godoy¹  Andrea Rocha Almeida Moraes¹  Marcos Doniseti Michelotto²  Namrata Mahrajan³  David John Bertioli⁴  Soraya Cristina de Macedo Leal-Bertioli⁵  Adriana Regina Custódio⁶  Márcio de Carvalho Moretzsohn⁶ 

[1] Grain and Fiber Center, Agronomic Institute of Campinas (IAC), Campinas, SP, Brazil;Grain and Fiber Center, Agronomic Institute of Campinas (IAC), Pindorama, SP, Brazil;Institute of Plant Breeding, Genetics and Genomics, University of Georgia, Athens, GA, United States;Institute of Plant Breeding, Genetics and Genomics, University of Georgia, Athens, GA, United States;Department of Crop and Soil Science, University of Georgia, Athens, GA, United States;Institute of Plant Breeding, Genetics and Genomics, University of Georgia, Athens, GA, United States;Department of Plant Pathology, University of Georgia, Athens, GA, United States;Plant Genetics Laboratory, Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil;

关键词: Arachis stenosperma;    A. magna;    induced allotetraploid;    late leaf spot;    rust;    molecular breeding;   

DOI  :  10.3389/fpls.2023.1139361

 received in 2023-01-06, accepted in 2023-02-20,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionFungal foliar diseases can severely affect the productivity of the peanut crop worldwide. Late leaf spot is the most frequent disease and a major problem of the crop in Brazil and many other tropical countries. Only partial resistance to fungal diseases has been found in cultivated peanut, but high resistances have been described on the secondary gene pool.MethodsTo overcome the known compatibility barriers for the use of wild species in peanut breeding programs, we used an induced allotetraploid (Arachis stenosperma × A. magna)4x, as a donor parent, in a successive backcrossing scheme with the high-yielding Brazilian cultivar IAC OL 4. We used microsatellite markers associated with late leaf spot and rust resistance for foreground selection and high-throughput SNP genotyping for background selection.ResultsWith these tools, we developed agronomically adapted lines with high cultivated genome recovery, high-yield potential, and wild chromosome segments from both A. stenosperma and A. magna conferring high resistance to late leaf spot and rust. These segments include the four previously identified as having QTLs (quantitative trait loci) for resistance to both diseases, which could be confirmed here, and at least four additional QTLs identified by using mapping populations on four generations.DiscussionThe introgression germplasm developed here will extend the useful genetic diversity of the primary gene pool by providing novel wild resistance genes against these two destructive peanut diseases.

【 授权许可】

Unknown   
Copyright © 2023 Moretzsohn, Santos, Moraes, Custódio, Michelotto, Mahrajan, Leal-Bertioli, Godoy and Bertioli