Comparative transcriptome analysis reveals the molecular mechanism of heat-tolerance in Neopyropia yezoensis induced by Sargassum horneri extract

Frontiers in Marine Science

Marine Science

Charles Yarish¹  Qikun Xing²  Sol Han²  Jang K. Kim³  Ji-Sook Park³ 

[1] Department of Ecology and Evolutionary Biology, University of Connecticut, Stamford, CT, United States;Department of Marine Science, Incheon National University, Incheon, Republic of Korea;Department of Marine Science, Incheon National University, Incheon, Republic of Korea;Research Institute of Basic Sciences, Incheon National University, Incheon, Republic of Korea;

关键词: sargassum horneri;    neopyropia yezoensis;    biostimulant;    heat-tolerance;    transcriptome;   

DOI  :  10.3389/fmars.2023.1142483

 received in 2023-01-11, accepted in 2023-03-01,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionHeat stress is one of the main threats for the seaweed aquaculture, leading to the decay of the blades and causing serious economic losses. A newly developed biostimulant, Sargassum horneri extract (SHE), can enhance the heat-tolerance in Neopyropia yezoensis, which can be applied in the seaweed aquaculture. However, the molecular mechanism underlying this acquired heat-tolerance is still unclear.MethodIn this study, we examined the transcriptional regulation of N. yezoensis under heat stress after the SHE pre-treatment by performing the RNA-Seq on illumina Novaseq platform and differentially expressed gene analysis.Result and discussionOur results show that, under heat stress, the SHE pre-treatment can alleviate the suppression of genes involved in normal cell activities, such as ribosome biogenesis, photosynthesis, respiration and cell cycle. On the other hand, SHE pre-treatment can also induce some key genes involved in the heat-tolerance. We also found that the combination of SHE pre-treatment and heat stress can induce some genes related to epigenetic modification, suggesting that SHE may facilitate the formation of heat stress memory in N. yezoensis. Our study provides information for understanding the molecular mechanism of heat-tolerance in N. yezoensis and for promoting the application of seaweed-derived biostimulants in the seaweed aquaculture.

【 授权许可】

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Copyright © 2023 Xing, Han, Park, Yarish and Kim