Integrated transcriptome and metabolome analysis unveil the response mechanism in wild rice (Zizania latifolia griseb.) against sheath rot infection

Frontiers in Genetics

Genetics

Yamin Ma¹  Quancong Wu²  Yiming Pan²  Dayun Zhou²  Tianjun He²  Lailiang Wang²  Limin Chen³  Yaobin Lu⁴  Xiaowei Li⁴  TingTing Chen⁵ 

[1] Agricultural and Rural Bureau of Jinyun County, Jinyun, Zhejiang, China;Lishui Institute of Agriculture and Forestry Sciences, Lishui, Zhejiang, China;Lishui Institute of Agriculture and Forestry Sciences, Lishui, Zhejiang, China;Ministry of Agriculture and Rural Affairs, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China;Ministry of Agriculture and Rural Affairs, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China;Ministry of Agriculture and Rural Affairs, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China;College of Plant Protection, Nanjing, China;

关键词: differentially accumulated metabolites;    differentially expressed genes;    Fusarium asiaticum;    hypersensitive response;    reactive oxygen species scavenging;   

DOI  :  10.3389/fgene.2023.1163464

 received in 2023-02-10, accepted in 2023-05-30,  发布年份 2023

来源: Frontiers

【 摘 要 】

Sheath rot disease (SRD) is one of the most devastating diseases of Manchurian wild rice (MWR) (Zizania latifolia Griseb). Pilot experiments in our laboratory have shown that an MWR cultivar “Zhejiao NO.7”exhibits signs of SRD tolerance. To explore the responses of Zhejiao No. 7 to SRD infection, we used a combined transcriptome and metabolome analysis approach. A total of 136 differentially accumulated metabolites (DAMs, 114 up- and 22 down-accumulated in FA compared to CK) were detected. These up-accumulated metabolites were enriched in tryptophan metabolism, amino acid biosynthesis, flavonoids, and phytohormone signaling. Transcriptome sequencing results showed the differential expression of 11,280 genes (DEGs, 5,933 up-, and 5,347 downregulated in FA compared to CK). The genes expressed in tryptophan metabolism, amino acid biosynthesis, phytohormone biosynthesis and signaling, and reactive oxygen species homeostasis confirmed the metabolite results. In addition, genes related to the cell wall, carbohydrate metabolism, and plant-pathogen interaction (especially hypersensitive response) showed changes in expression in response to SRD infection. These results provide a basis for understanding the response mechanisms in MWR to FA attack that can be used for breeding SRD-tolerant MWR.

【 授权许可】

Unknown   
Copyright © 2023 Chen, Ma, He, Chen, Pan, Zhou, Li, Lu, Wu and Wang.