Probiotic Escherichia coli Nissle 1917-derived outer membrane vesicles modulate the intestinal microbiome and host gut-liver metabolome in obese and diabetic mice

Frontiers in Microbiology

Microbiology

Richard D. Cannon¹  XingYu Pu²  Fei Long²  Jun Shi²  DongXue Ma²  Xin Wang²  ShanHu Gao²  Ting-Li Han³ 

[1] Department of Oral Sciences, Faculty of Dentistry, Sir John Walsh Research Institute, University of Otago, Dunedin, New Zealand;State Key Laboratory of Ultrasound in Medicine and Engineering, College of Biomedical Engineering, Chongqing Medical University, Chongqing, China;Chongqing Key Laboratory of Biomedical Engineering, Chongqing Medical University, Chongqing, China;State Key Laboratory of Ultrasound in Medicine and Engineering, College of Biomedical Engineering, Chongqing Medical University, Chongqing, China;Chongqing Key Laboratory of Biomedical Engineering, Chongqing Medical University, Chongqing, China;Department of Obstetrics and Gynaecology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China;

关键词: probiotics;    Escherichia coli;    outer membrane vesicles;    diabetes;    obesity;    gut microbiota;    metabolomics;   

DOI  :  10.3389/fmicb.2023.1219763

 received in 2023-05-09, accepted in 2023-07-26,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionObesity and diabetes are common chronic metabolic disorders which can cause an imbalance of the intestinal flora and gut-liver metabolism. Several studies have shown that probiotics, including Escherichia coli Nissle 1917 (EcN), promote microbial balance and metabolic health. However, there are no studies on how EcN outer membrane vesicles (EcN-OMVs) influence the intestinal microflora and affect the metabolic disorders of obesity and diabetes.MethodsIn this study, we evaluated the effects of EcN-OMVs on high-fat diet (HFD)-induced obesity and HFD + streptozotocin (STZ)-induced diabetes.ResultsEcN-OMVs could reduce body weight, decrease blood glucose, and increase plasma insulin in obese mice. Similarly, EcN-OMVs treatment could modify the ratio of Firmicutes/Bacteroidetes in the gut, elevate intestinal short-chain fatty acid (SCFA)-producing flora, and influence the SCFA content of the intestine. Furthermore, the intestinal metabolites ornithine and fumaric acid, hepatic ω-6 unsaturated fatty acids, and SCFAs were significantly increased after administering EcN-OMVs.DiscussionOverall, this study showed that EcN-OMVs might act as post-biotic agents that could modulate gut-liver metabolism and ameliorate the pathophysiology of obesity and diabetes.

【 授权许可】

Unknown   
Copyright © 2023 Shi, Ma, Gao, Long, Wang, Pu, Cannon and Han.