Do temperature and population size structure influence the quantity of fish eDNA in water?

Frontiers in Environmental Science

Environmental Science

Pierre Gibert¹  Maxime Logez²  Isabelle Domaizon³  Jean-Marc Baudoin⁴  Tony Dejean⁵  Alice Valentini⁵  Alix Hervé⁶ 

[1] Pole R&D ECLA, Aix-en-Provence, France;INRAE, Aix Marseille Université, RECOVER, France;Pole R&D ECLA, Aix-en-Provence, France;INRAE, Aix Marseille Université, RECOVER, France;INRAE, UR RIVERLY, Villeurbanne Cedex, France;Pole R&D ECLA, Aix-en-Provence, France;INRAE, UMR CARRTEL, Thonon-les-Bains, France;Pole R&D ECLA, Aix-en-Provence, France;OFB, Direction de la Recherche et de l’Appui Scientifique, Aix-en-Provence, France;SPYGEN, Le Bourget-du-Lac, France;SPYGEN, Le Bourget-du-Lac, France;Pole R&D ECLA, Aix-en-Provence, France;INRAE, Aix Marseille Université, RECOVER, France;

关键词: mesocosm;    Tinca tinca;    eDNA quantification;    teleo;    metabarcoding;   

DOI  :  10.3389/fenvs.2023.1193393

 received in 2023-03-24, accepted in 2023-07-14,  发布年份 2023

来源: Frontiers

【 摘 要 】

Various factors can influence the concentration of eDNA found in the environment and blur the link between eDNA results and in situ fish abundances, biomasses or even occurrences. We studied the influence of one abiotic factor, the temperature, and one biotic factor, the fish size class, on the amount of fish eDNA detectable in water. To do so, we conducted two controlled experiments using tench (Tinca tinca, L) placed into outdoor mesocosms during 1 week. The quantification of fish eDNA abundances was performed using quantitative PCR after filtrating a large volume of water (30 L). The Bayesian hierarchical ANOVAs performed on qPCR results did not detect the effect of size class on eDNA amount, but fish eDNA concentration was shown to increase significantly when temperature rose by 6°C (no effect detected at 3°C). This suggests that fish assemblage quantification of two thermally contrasting sites through eDNA is not directly comparable.

【 授权许可】

Unknown   
Copyright © 2023 Hervé, Domaizon, Baudoin, Dejean, Gibert, Valentini and Logez.