Mapping of a QTL associated with sucrose content in peanut kernels using BSA-seq

Frontiers in Genetics

Genetics

Xiaona Li¹  Jing Xu¹  Chenyu Li¹  Mengyuan Zhang¹  Feiyan Qi¹  Qi Zhao¹  Dandan Luo¹  Hua Liu¹  Junjia Guo¹  Ziqi Sun¹  Hongyan Li¹  Wenzhao Dong¹  Lijuan Miao¹  Bingyan Huang¹  Mengjie Cui¹  Maoning Zhang¹  Mengdi Tian¹  Li Qin¹  Xinyou Zhang²  Suoyi Han³ 

[1] Henan Academy of Crops Molecular Breeding/Postgraduate T&R Base of Zhengzhou University/The Shennong Laboratory/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crop Improvement/National Centre for Plant Breeding, Xinxiang, Henan, China;null;[e-mail];

关键词: peanut;    sucrose content;    BSA-seq;    QTL;    KASP;   

DOI  :  10.3389/fgene.2022.1089389

 received in 2022-11-04, accepted in 2022-11-28,  发布年份 2023

来源: Frontiers

【 摘 要 】

As an important factor affecting the edible quality of peanut kernels, sucrose content is a complex quantitative trait regulated by multiple factors. In this study, an F2 segregating population and a recombinant inbred line (RIL) population, derived from a cross between the high sucrose content variety Jihuatian 1 and the low sucrose content line PI478819, were used as materials to map a quantitative trait locus (QTL) associated with sucrose content in peanut kernels. Four QTLs were initially located on chromosomes A03 and A06 based on BSA-seq technology, and multiple kompetitive allele-specific PCR markers were developed based on single-nucleotide polymorphisms (SNPs) in the intervals. The markers were genotyped in the RIL population and finely mapped to a stable QTL, qSUCA06, located on chromosome A06 within a 0.29-Mb physical genomic interval (112367085–112662675 bp), which accounted for 31.95%–41.05% of the phenotypic variance explained. SNP and insertion/deletion annotations were performed on genes in the candidate interval, and having screened out those genes with mutations in exons, candidate genes were verified by qRT-PCR. The results revealed that Arahy.Y2LWD9 may be the main gene regulating sucrose content. The QTL identified in this study will not only contribute to marker-assisted breeding for improvement of peanut sucrose content but also paves the way for identifying gene function.

【 授权许可】

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Copyright © 2023 Guo, Qi, Qin, Zhang, Sun, Li, Cui, Zhang, Li, Li, Zhao, Luo, Tian, Liu, Xu, Miao, Huang, Dong, Han and Zhang.