Field test of Easter lilies transformed with a rice cystatin gene for root lesion nematode resistance

Frontiers in Plant Science

Plant Science

Becky Westerdahl¹  Lee Riddle²  Kathryn Kamo³  Deborah Giraud⁴ 

[1] Department of Entomology and Nematology, University of California, Davis, Davis, CA, United States;Easter Lily Research Foundation, Brookings, OR, United States;Floral & Nursery Plants Research Unit, United States Department of Agriculture (USDA), Beltsville, MD, United States;University of California Cooperative Extension, Eureka, CA, United States;

关键词: Pratylenchus penetrans;    cysteine protease;    Lilium longiflorum;    nematode management;    pesticide use;   

DOI  :  10.3389/fpls.2023.1134224

 received in 2022-12-30, accepted in 2023-03-06,  发布年份 2023

来源: Frontiers

【 摘 要 】

Easter lilies, Lilium longiflorum cv. Nellie White are a staple of the floral industry. In the U.S. most of the Easter lilies are grown in Oregon and California along the coast where there is a micro climate that is favorable to growth of lilies. The main pest when growing lilies in the field is Pratylenchus penetrans, the root lesion nematode. Easter lilies are one of the most expensive crops to produce because of the cost of chemicals used to control P. penetrans and other pathogens that infect the lilies. Our previous study had shown that transgenic Easter lilies containing a rice cystatin gene (Oc-IΔD86 that has a deleted Asp86) were resistant to P. penetrans in vitro. This study examined growth characteristics of five independently transformed lines of the cystatin Easter lilies compared to non-transformed Nellie White for three seasons in the field in Brookings, Oregon. Liles grown in three soil chemical treatments 1) preplant fumigation, 2) preplant fumigation plus at plant organophosphate, and 3) at plant organophosphate were compared to those grown in nontreated soil. Growth characteristics evaluated included: time of shoot emergence, survival of plants, size of plants, visual ratings of plant health, basal roots and stem roots, weight of foliage and roots, and number and size of bulblets that developed on stems. Nematodes were counted following their extraction from the roots. While not totally resistant, when planted in the field, transformed lines demonstrated and maintained a degree of resistance to lesion nematode over two growing seasons and displayed desirable growth and quality characteristics similar to non-transformed lilies.

【 授权许可】

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Copyright © 2023 Westerdahl, Riddle, Giraud and Kamo