ECT9 condensates with ECT1 and regulates plant immunity

Frontiers in Plant Science

Plant Science

Ruixia Niu¹  Hui Wang¹  Yulu Zhou¹  Guilong Zhou¹  Zhijuan Tang¹  Guoyong Xu² 

[1]State Key Laboratory of Hybrid Rice, Institute for Advanced Studies (IAS), Wuhan University, Wuhan, Hubei, China

[2]State Key Laboratory of Hybrid Rice, Institute for Advanced Studies (IAS), Wuhan University, Wuhan, Hubei, China

[3]Hubei Hongshan Laboratory, Wuhan, Hubei, China

关键词: RNA binding proteins;    YT521-B homology domain;    ECT9;    ECT1;    Liquid-liquid phase separation;    Condensation;    Plant immunity;    Arabidopsis thaliana;   

DOI  :  10.3389/fpls.2023.1140840

 received in 2023-01-09, accepted in 2023-03-28,  发布年份 2023

来源: Frontiers

【 摘 要 】

Mounting an efficient defense against pathogens requires RNA binding proteins (RBPs) to regulate immune mRNAs transcription, splicing, export, translation, storage, and degradation. RBPs often have multiple family members, raising the question of how they coordinate to carry out diverse cellular functions. In this study, we demonstrate that EVOLUTIONARILY CONSERVED C-TERMINAL REGION 9 (ECT9), a member of the YTH protein family in Arabidopsis, can condensate with its homolog ECT1 to control immune responses. Among the 13 YTH family members screened, only ECT9 can form condensates that decrease after salicylic acid (SA) treatment. While ECT1 alone cannot form condensates, it can be recruited to ECT9 condensates in vivo and in vitro. Notably, the ect1/9 double mutant, but not the single mutant, exhibits heightened immune responses to the avirulent pathogen. Our findings suggest that co-condensation is a mechanism by which RBP family members confer redundant functions.

【 授权许可】

Unknown   
Copyright © 2023 Wang, Niu, Zhou, Tang, Xu and Zhou