Root Morphogenesis of Arabidopsis thaliana Tuned by Plant Growth-Promoting Streptomyces Isolated From Root-Associated Soil of Artemisia annua

Frontiers in Plant Science

Plant Science

Daozhi Gong¹  Dongfei Han¹  Yuzhong Li¹  Yanshuo Pan²  Yuhua Shi³  Guangfei Hao⁴  Wenbo Fu⁵  Jieyin Chen⁶ 

[1] Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing, China;Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing, China;College of Natural Resources and Environment, Northwest Agriculture and Forestry University, Yangling, China;Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Material Medica, China Academy of Chinese Medical Sciences, Beijing, China;School of Life Sciences and Food Engineering, Hebei University of Engineering, Handan, China;School of Life Sciences and Food Engineering, Hebei University of Engineering, Handan, China;Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing, China;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China;

关键词: Streptomyces lincolnensis;    PGPR;    indole-3-acetic acid (IAA);    root morphogenesis;    endophytic microbes;   

DOI  :  10.3389/fpls.2021.802737

 received in 2021-10-27, accepted in 2021-11-25,  发布年份 2022

来源: Frontiers

【 摘 要 】

In this study, the capacity to tune root morphogenesis by a plant growth-promoting rhizobacterium, Streptomyces lincolnensis L4, was investigated from various aspects including microbial physiology, root development, and root endophytic microbial community. Strain L4 was isolated from the root-associated soil of 7-year plantation of Artemisia annua. Aiming at revealing the promotion mechanism of Streptomyces on root growth and development, this study first evaluated the growth promotion characters of S. lincolnensis L4, followed by investigation in the effect of L4 inoculation on root morphology, endophytic microbiota of root system, and expression of genes involved in root development in Arabidopsis thaliana. Streptomyces lincolnensis L4 is able to hydrolyze organic and inorganic phosphorus, fix nitrogen, and produce IAA, ACC deaminase, and siderophore, which shaped specific structure of endophytic bacterial community with dominant Streptomyces in roots and promoted the development of roots. From the observation of root development characteristics, root length, root diameter, and the number of root hairs were increased by inoculation of strain L4, which were verified by the differential expression of root development-related genes in A. thaliana. Genomic traits of S. lincolnensis L4 which further revealed its capacity for plant growth promotion in which genes involved in phosphorus solubilization, ACC deamination, iron transportation, and IAA production were identified. This root growth-promoting strain has the potential to develop green method for regulating plant development. These findings provide us ecological knowledge of microenvironment around root system and a new approach for regulating root development.

【 授权许可】

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Copyright © 2022 Fu, Pan, Shi, Chen, Gong, Li, Hao and Han.