Exposure to Dichlorvos pesticide alters the morphology of and lipid metabolism in the ventral prostate of rats

Frontiers in Toxicology

Toxicology

Naíra Ruiz Lenharo¹  Laura Ribeiro da Silva¹  Cristiane Figueiredo Pinho¹  Vinícius Luís Rocha da Silva Maria¹  Giovanna Galo Quintino-Ottonicar¹  Eleonora Malavolta Pizzo¹  Ana Clara Pacheco de Santana¹  Sergio Pereira² 

[1]Endocrine Disruptors Laboratory, School of Sciences, Department of Biological Sciences, São Paulo State University, Bauru, Brazil

[2]null

关键词: Dichlorvos;    lipid metabolism;    SREBP;    SCAP;    LIMP-II;    CD36;    prostate cancer;   

DOI  :  10.3389/ftox.2023.1207612

 received in 2023-04-17, accepted in 2023-06-22,  发布年份 2023

来源: Frontiers

【 摘 要 】

Organophosphate pesticides are widely used in agriculture, leading to soil, water, and food contamination. Among these compounds is Dichlorvos [O,O-dimethyl O-(2,2-dichlorovinyl)phosphate, DDVP], which is listed as a highly toxic compound by the Environmental Protection Agency and World Health Organization. Exposure to DDVP can result in nervous, respiratory, hepatic, and reproductive abnormalities, in addition to endocrine disrupting, mutagenic, and carcinogenic effects. Little is known about the impacts of DDVP on the reprogramming of lipid metabolism, which is also associated with the development and progression of cancer, since the tumor cells need to recruit, capture, and use fatty acids to compose their building membranes. This study aimed to evaluate the influence of the pesticide DDVP on lipid metabolism in the prostate, after chemical induction by the carcinogen N-methyl-N-nitrosourea (MNU). For this, 32 Fischer rats aged 90 days were randomly divided into four experimental groups: Control, DDVP, MNU, and MNU + DDVP. The MNU and MNU + DDVP groups underwent chemical induction with MNU (15 mg/kg) and the DDVP and MNU + DDVP groups received a diet supplemented with DDVP (10 mg/kg). Histopathological analyses of the rat ventral prostate showed 100% incidence of epithelial hyperplasia in the MNU and MNU + DDVP groups. This finding was accompanied by an increase of the epithelial compartment in the MNU + DDVP group. Immunolocalization of important proteins linked to lipid metabolism has been established. In the MNU + DDVP group, Western blotting analyses pointed out an increased expression of the protein LIMP II (Lysosomal Integral Membrane Protein-II), which is correlated with the capture and distribution of lipids in tumor cells. Together, these results indicate that the association of a low dose of DDVP with MNU was able to promote alterations in the morphology and lipid metabolism of the rat ventral prostate, which may be related to tumor progression in this organ.

【 授权许可】

Unknown   
Copyright © 2023 Quintino-Ottonicar, Silva, Maria, Pizzo, Santana, Lenharo, Pinho and Pereira.