Tracing the evolutionary history of hepatitis B virus genotype H endemic to Mexico

Frontiers in Microbiology

Microbiology

João Renato Rebello-Pinho¹  Sonia Roman²  Alexis Jose-Abrego²  Arturo Panduro²  Saul Laguna-Meraz³  Santiago Justo Arevalo⁴ 

[1] Department of Gastroenterology, Institute of Tropical Medicine and School of Medicine, LIM07, University of São Paulo, São Paulo, Brazil;Hospital Israelita Albert Einstein, São Paulo, Brazil;Department of Genomic Medicine in Hepatology, Civil Hospital of Guadalajara, "Fray Antonio Alcalde", Guadalajara, Jalisco, Mexico;Health Sciences Center, University of Guadalajara, Guadalajara, Jalisco, Mexico;Department of Genomic Medicine in Hepatology, Civil Hospital of Guadalajara, "Fray Antonio Alcalde", Guadalajara, Jalisco, Mexico;Health Sciences Center, University of Guadalajara, Guadalajara, Jalisco, Mexico;Molecular Biology in Medicine Doctorate Program, Guadalajara, Mexico;Faculty of Biological Sciences, Ricardo Palma University, Lima, Peru;Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, Brazil;

关键词: hepatitis B virus;    Mexico;    origin;    evolution;    genotype H;    Mesoamerica;    indigenous populations;   

DOI  :  10.3389/fmicb.2023.1180931

 received in 2023-03-06, accepted in 2023-05-02,  发布年份 2023

来源: Frontiers

【 摘 要 】

Hepatitis B virus (HBV) spreads efficiently among all human populations worldwide. HBV is classified into ten genotypes (A to J) with their geographic distribution and clinical features. In Mexico, HBV genotype H is the leading cause of hepatitis B and has been detected in indigenous populations, suggesting that HBV genotype H may be native to Mexico. However, little is known about the evolutionary history of HBV genotype H. Thus, we aimed to determine the age of HBV genotype H in Mexico using molecular dating techniques. Ninety-two HBV sequences of the reverse transcriptase (RT) domain of the polymerase gene (~1,251 bp) were analyzed; 48 were genotype H, 43 were genotype F, and the oldest HBV sequence from America was included as the root. All sequences were aligned, and the most recent common ancestor (TMRCA) time was calculated using the Bayesian Skyline Evolutionary Analysis. Our results estimate a TMRCA for the genotype H in Mexico of 2070.9 (667.5–4489.2) years before the present (YBP). We identified four major diversification events in genotype H, named H1, H2, H3, and H4. The TMRCA of H1 was 1213.0 (253.3–2638.3) YBP, followed by H2 1175.5 (557.5–2424.2) YBP, H3 949.6 (279.3–2105.0) YBP, and H4 1230.5 (336.3, 2756.7) YBP. We estimated that genotype H diverged from its sister genotype F around 8140.8 (1867.5–18012.8) YBP. In conclusion, this study found that genotype H in Mexico has an estimated age of 2070.9 (667.5–4489.2) YBP and has experienced at least four major diversification events since then.

【 授权许可】

Unknown   
Copyright © 2023 Jose-Abrego, Roman, Laguna-Meraz, Rebello-Pinho, Justo Arevalo and Panduro.