Tailoring the HHx monomer content of P(HB-co-HHx) by flexible substrate compositions: scale-up from deep-well-plates to laboratory bioreactor cultivations

Frontiers in Bioengineering and Biotechnology

Bioengineering and Biotechnology

Peter Neubauer¹  Lara Santolin¹  Isabel Thiele¹  Sebastian L. Riedel² 

[1] Technische Universität Berlin, Institute of Biotechnology, Chair of Bioprocess Engineering, Berlin, Germany;Technische Universität Berlin, Institute of Biotechnology, Chair of Bioprocess Engineering, Berlin, Germany;Berliner Hochschule für Technik, Department VIII – Mechanical Engineering, Event Technology and Process Engineering, Laboratory of Environmental and Bioprocess Engineering, Berlin, Germany;

关键词: bioplastic;    PHA;    polyhydroxyalkanoate;    co;    Ralstonia eutropha;    substrate-flexible;    biodegradable;    scale-up;   

DOI  :  10.3389/fbioe.2023.1081072

 received in 2022-10-26, accepted in 2023-04-18,  发布年份 2023

来源: Frontiers

【 摘 要 】

The enhanced material properties exhibited by the microbially synthetized polyhydroxyalkanoate (PHA) copolymer poly(hydroxybutyrate-co-hydroxyhexanoate) [P(HB-co-HHx)] evidence that this naturally biodegrading biopolymer could replace various functionalities of established petrochemical plastics. In fact, the thermal processability, toughness and degradation rate of P(HB-co-HHx) can be tuned by modulating its HHx molar content enabling to manufacture polymers à-la-carte. We have developed a simple batch strategy to precisely control the HHx content of P(HB-co-HHx) to obtain tailor-made PHAs with defined properties. By adjusting the ratio of fructose to canola oil as substrates for the cultivation of recombinant Ralstonia eutropha Re2058/pCB113, the molar fraction of HHx in P(HB-co-HHx) could be adjusted within a range of 2–17 mol% without compromising polymer yields. The chosen strategy proved to be robust from the mL-scale in deep-well-plates to 1-L batch bioreactor cultivations.

【 授权许可】

Unknown   
Copyright © 2023 Santolin, Thiele, Neubauer and Riedel.