CRISPR-cas technology: A key approach for SARS-CoV-2 detection

Frontiers in Bioengineering and Biotechnology

Bioengineering and Biotechnology

Huimei Xu¹  Lijuan Fang¹  Lusen Yang¹  Mingyue Han¹  Wenshuai Ding¹  Xuejun Dong² 

[1] Department of Laboratory Medicine, Hangzhou Ninth People’s Hospital, Hangzhou, Zhejiang Province, China;Medical Laboratory, Zhejiang University Shaoxing Hospital, Shaoxing, China;

关键词: CRISPR-Cas;    nucleic acid detection;    SARS-CoV-2;    application;    COVID-19;   

DOI  :  10.3389/fbioe.2023.1158672

 received in 2023-02-09, accepted in 2023-03-31,  发布年份 2023

来源: Frontiers

【 摘 要 】

The CRISPR (Clustered Regularly Spaced Short Palindromic Repeats) system was first discovered in prokaryotes as a unique immune mechanism to clear foreign nucleic acids. It has been rapidly and extensively used in basic and applied research owing to its strong ability of gene editing, regulation and detection in eukaryotes. Hererin in this article, we reviewed the biology, mechanisms and relevance of CRISPR-Cas technology and its applications in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) diagnosis. CRISPR-Cas nucleic acid detection tools include CRISPR-Cas9, CRISPR-Cas12, CRISPR-Cas13, CRISPR-Cas14, CRISPR nucleic acid amplification detection technology, and CRISPR colorimetric readout detection system. The above CRISPR technologies have been applied to the nucleic acid detection, including SARS-CoV-2 detection. Common nucleic acid detection based on CRISPR derivation technology include SHERLOCK, DETECTR, and STOPCovid. CRISPR-Cas biosensing technology has been widely applied to point-of-care testing (POCT) by targeting recognition of both DNA molecules and RNA Molecules.

【 授权许可】

Unknown   
Copyright © 2023 Fang, Yang, Han, Xu, Ding and Dong.