Genome-wide association analysis provides insights into the genetic basis of photosynthetic responses to low-temperature stress in spring barley

Frontiers in Plant Science

Plant Science

Jan J. Slaski¹  Aaron D. Beattie²  Guillermo Hernandez Ramirez³  Ludovic J.A. Capo-chichi³  Ammar Elakhdar⁴  Takahiko Kubo⁵  Aladdin Hamwieh⁶ 

[1] Bio Industrial Services Division, InnoTech Alberta Inc., Vegreville, AB, Canada;Department of Plant Sciences, College of Agriculture and Bioresources, University of Saskatchewan, Saskatoon, SK, Canada;Department of Renewable Resources, Faculty of Agriculture, Life and Environmental Sciences, University of Alberta, Edmonton, AB, Canada;Field Crops Research Institute, Agricultural Research Center, Giza, Egypt;Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Fukuoka, Japan;Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Fukuoka, Japan;International Center for Agriculture Research in the Dry Areas (ICARDA), Giza, Egypt;

关键词: Hordeum vulgare;    chlorophyll fluorescence;    photosystem II photochemistry;    quantitative trait nucleotides (QTNs);    Mixed linear model (MLM);    Abscisic acid (ABA) signaling;    protein kinase;    post-transcription modification;   

DOI  :  10.3389/fpls.2023.1159016

 received in 2023-02-05, accepted in 2023-05-04,  发布年份 2023

来源: Frontiers

【 摘 要 】

Low-temperature stress (LTS) is among the major abiotic stresses affecting the geographical distribution and productivity of the most important crops. Understanding the genetic basis of photosynthetic variation under cold stress is necessary for developing more climate-resilient barley cultivars. To that end, we investigated the ability of chlorophyll fluorescence parameters (FVFM, and FVF0) to respond to changes in the maximum quantum yield of Photosystem II photochemistry as an indicator of photosynthetic energy. A panel of 96 barley spring cultivars from different breeding zones of Canada was evaluated for chlorophyll fluorescence-related traits under cold acclimation and freeze shock stresses at different times. Genome-wide association studies (GWAS) were performed using a mixed linear model (MLM). We identified three major and putative genomic regions harboring 52 significant quantitative trait nucleotides (QTNs) on chromosomes 1H, 3H, and 6H for low-temperature tolerance. Functional annotation indicated several QTNs were either within the known or close to genes that play important roles in the photosynthetic metabolites such as abscisic acid (ABA) signaling, hydrolase activity, protein kinase, and transduction of environmental signal transduction at the posttranslational modification levels. These outcomes revealed that barley plants modified their gene expression profile in response to decreasing temperatures resulting in physiological and biochemical modifications. Cold tolerance could influence a long-term adaption of barley in many parts of the world. Since the degree and frequency of LTS vary considerably among production sites. Hence, these results could shed light on potential approaches for improving barley productivity under low-temperature stress.

【 授权许可】

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Copyright © 2023 Elakhdar, Slaski, Kubo, Hamwieh, Hernandez Ramirez, Beattie and Capo-chichi