Identification of intestinal microbiome associated with lymph-vascular invasion in colorectal cancer patients and predictive label construction

Frontiers in Cellular and Infection Microbiology

Cellular and Infection Microbiology

Weizhong Tang¹  Chenyan Long¹  Xianwei Mo¹  Binzhe Tang¹  Chuanbin Chen¹  Kang Chen¹  Xiaoliang Huang¹  Zigui Huang¹  Zhen Wang¹  Mingjian Qin¹  Jungang Liu¹  Fuhai He¹ 

[1] Division of Colorectal & Anal Surgery, Department of Gastrointestinal Surgery, Guangxi Medical University Cancer Hospital, Nanning, China;

关键词: colorectal cancer;    lymph-vascular invasion;    16S rRNA;    intestinal microbiome;    machine learning;   

DOI  :  10.3389/fcimb.2023.1098310

 received in 2022-11-14, accepted in 2023-04-04,  发布年份 2023

来源: Frontiers

【 摘 要 】

ObjectiveTo identify differences between the composition, abundance, and biological function of the intestinal microbiome of patients with and without lymph-vascular invasion (LVI) colorectal cancer (CRC) and to construct predictive labels to support accurate assessment of LVI in CRC.Method134 CRC patients were included, which were divided into two groups according to the presence or absence of LVI, and their intestinal microbiomes were sequenced by 16SrRNA and analyzed for differences. The transcriptome sequencing data of 9 CRC patients were transformed into immune cells abundance matrix by CIBERSORT algorithm, and the correlation among LVI-associated differential intestinal microbiomes, immune cells, immune-related genes and LVI-associated differential GO items and KEGG pathways were analyzed. A random forest (RF) and eXtreme Gradient Boosting (XGB) model were constructed to predict the LVI of CRC patients based on the differential microbiome.ResultThere was no significant difference in α-diversity and β-diversity of intestinal microbiome between CRC patients with and without LVI (P > 0.05). Linear discriminant analysis Effect Size (LEfSe) analysis showed 34 intestinal microbiomes enriched in CRC patients of the LVI group and 5 intestinal microbiomes were significantly enriched in CRC patients of the non-lymph-vascular invasion (NLVI) group. The RF and XGB prediction models constructed with the top 15% of the LVI-associated differential intestinal microbiomes ranked by feature significance had good efficacy.ConclusionsThere are 39 intestinal flora with significantly different species abundance between the LVI and NLVI groups. g:Alistipes.s:Alistipes_indistinctus is closely associated with colorectal cancer vascular invasion. LVI-associated differential intestinal flora may be involved in regulating the infiltration of immune cells in CRC and influencing the expression of immune-related genes. LVI-associated differential intestinal flora may influence the process of vascular invasion in CRC through a number of potential biological functions. RF prediction models and XGB prediction models constructed based on microbial markers of gut flora can be used to predict CRC-LVI conditions.

【 授权许可】

Unknown   
Copyright © 2023 Chen, Chen, Huang, Huang, Wang, He, Qin, Long, Tang, Mo, Liu and Tang