Multiplex analysis of cytokines in the cerebrospinal fluid of dogs after ischemic stroke reveals elevations in chemokines CXCL1 and MCP-1

Frontiers in Veterinary Science

Veterinary Science

Jamie Barber¹  Simon R. Platt²  Renee M. Barber²  Kelsey R. Robinson²  Luisa De Risio³ 

[1] Department of Infectious Diseases, University of Georgia College of Veterinary Medicine, Athens, GA, United States;Department of Small Animal Medicine and Surgery, University of Georgia College of Veterinary Medicine, Athens, GA, United States;Linnaeus Veterinary Limited, Shirley, United Kingdom;

关键词: dog;    stroke;    cerebrovascular accident;    cerebrospinal fluid;    cytokine;    chemokine;    CXCL1;    MCP-1;   

DOI  :  10.3389/fvets.2023.1169617

 received in 2023-02-19, accepted in 2023-04-24,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionNeuroinflammation that occurs in the brain after stroke has been shown to be important to disease pathogenesis and outcomes. The aim of this study was to evaluate a large number of pro- and anti-inflammatory cytokines in dogs with clinically-confirmed, naturally occurring stroke.Materials and methodsFifteen dogs with a clinical diagnosis of ischemic stroke and ten healthy control dogs were included in the study. A multiplex immunoassay was utilized to evaluate cerebrospinal fluid for GM-CSF, IFN-γ, IL-2, IL-4, IL-6, IL-7, IL-8, IL-10, IL-15, IL-18, IP-10, CXCL1, MCP-1, and TNF-α.ResultsMean concentrations of CXCL1 (stroke-436 pg/ml, control-267 pg/ml, p = 0.01) and MCP-1 (stroke-196 pg/ml, control-66 pg/ml, p ≤ 0.0001) were significantly elevated in dogs with stroke when compared with control dogs. Location and type of infarct, duration of clinical signs, and use of anti-inflammatory medications were not associated with differences in cytokine concentration.DiscussionCXCL1 and MCP-1 may play a role in naturally occurring canine stroke and represent targets for future research.

【 授权许可】

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Copyright © 2023 Barber, Platt, De Risio, Barber and Robinson.